褐藻多糖对氧化应激诱导的淋巴细胞DNA损伤的保护作用  

THE PROTECTIVE EFFECT OF THE SULFATED POLYSACCHARIDES FROM BROWN SEAWEED ON LYMPHOCYTE DNA DAMAGE CAUSED BY OXYGENRADIAL

作　　者：詹林盛[1] 张新生[1] 吴小红[1] 王颖丽[2] 王之贤[1] 

机构地区：[1]军事医学科学院卫生学与环境医学研究所,天津300050 [2]解放军第八十八医院检验科

出　　处：《中国海洋药物》1999年第4期4-7,共4页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金资助!(39770644)

摘　　要：研究褐藻多糖（ＢＳＰ）对活性氧引起的淋巴细胞ＤＮＡ损伤的保护作用。方法是敏感的单细胞凝胶电泳（ＳＣＧＥ）。结果表明在不同的介质中Ｈ_２Ｏ_２对细胞ＤＮＡ的损伤程度不同，在同一介质中Ｈ_２Ｏ_２对细胞ＤＮＡ的损伤程度呈一定的量效关系。 １００ｍｇ／Ｌ和 ２００ｍｇ／Ｌ的大分子量多糖（ＢＳＰｈ）和小分子量多糖（ＢＳＰｓ）都能明显降低Ｈ_２Ｏ_２对细胞ＤＮＡ损伤程度。表明ＢＳＰ能够清除自由基，减低氧化应激所致的细胞ＤＮＡ损伤程度，有利于维护细胞的正常结构和功能。提示 ＢＳＰ可作为一种预防性抗氧化营养素，用于预防氧化应激引起的疾病。The purpose of this study is to investigate the protective effects of sulfated polysaccharides (BSP)from brown seaweeds (large molecule BSPh and small molecule BSPs ) on oxygen-radial-generated DNA damage in human lymphocytes. A sensitive method-single cell gelelectrophoresis (SCGE)was used to observe the lymphocytes DNA damage. The re sults showed that in different mediums the DNA damage rate of human lymphocytes treated with H_2O_2 was different,but in the same medium the DNA damage grade of human lympho cytes had dose-effect relationships. BSPh and BSPs (100mg/L and 200mg/L)showed the pro tective effects,respectively. These results suggested that BSP might have the scavenging ef fects on radial produced by H_2O_2,and reduce the oxygen-radial-generated DNA damage in human lymphocytes,maintain normal structure and function of cells and be beneficial in pre vention for some diseases associated with oxidative stress.

关 键 词：褐藻多糖 单细胞凝胶电泳 DNA损伤 氧化应激 

分 类 号：R361[医药卫生—病理学] R977.6[医药卫生—基础医学]