氯霉素包合物的拉曼光谱研究  

Study on the Inclusion Compound of Chloramphenicol by Raman Spectroscopy

作　　者：王玮[1] 钱佩佩[1] 曹凯[1] 田京辉[1] 席欣欣[1] 李晓曼[1] 

机构地区：[1]河南大学药学院,河南开封475004

出　　处：《分析科学学报》2011年第6期805-807,共3页Journal of Analytical Science

摘　　要：通过拉曼光谱法研究氯霉素与β-环糊精相互作用形成包合物的过程,探讨拉曼光谱作为一种新的验证包合物形成方法的可行性。采用饱和水溶液法制备氯霉素包合物,共聚焦拉曼光谱仪分别检测氯霉素、β-环糊精、氯霉素与β-环糊精的物理混合物、氯霉素包合物的拉曼光谱并对照分析。结果表明,拉曼光谱法可说明氯霉素的苯环结构和易水解基团二氯乙酰胺基通过氢键作用,被包裹在β-环糊精的空穴结构中形成包合物,增加了它的稳定性和溶出度。该法具有快速、准确、简便、无损测量等显著优势,可作为验证药物包合物形成的重要手段。The inclusion action between chloramphenicol and β-cyclodextrins was studied using confocal Raman spectroscopy in order to evaluate the capability in identifying the inclusion compound.The Raman spectra of chloramphenicol,β-cyclodextrins,β-cyclodextrins and chloramphenicol physical mixture,chloramphenicol-β-cyclodextrins inclusion compound were obtained with confocal Raman spectrometer and then were compared.Raman characteristics shows that benzene ring structure and easily hydrolyzed structure of chloramphenicol dichloro-acetyl amino group are wrapped in the cavities of β-cyclodextrin structure through hydrogen bonds,increasing its stability and dissolution.The results show Raman spectroscopy has advantages such as rapid,accurate,simple and non-destructive in identifying the inclusion compound.

关 键 词：拉曼光谱法 氯霉素 Β-环糊精 包合物 

分 类 号：O657.37[理学—分析化学]