γ辐照对枯草芽孢杆菌营养体的损伤  

Damage Effect of γ-rays on Bacillus Subtilis Vegetetive Cells

作　　者：陈晓明[1] 柳芳[1] 郑春[2] 李晓燕[2] 张建国[1] 严万里[1] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]中国工程物理研究院核物理与化学研究所,四川绵阳621900

出　　处：《原子能科学技术》2011年第7期875-879,共5页Atomic Energy Science and Technology

基　　金：中国工程物理研究院科学技术发展基金资助项目(2008A0103002);西南科技大学核废物与环境安全国防重点学科实验室基金资助项目(07JGZB07);国家自然科学基金资助项目(11075134);绵阳市科技局资助项目(08S004-2)

摘　　要：选用不同剂量γ射线辐照枯草芽孢杆菌营养体,分别用细胞计数、黄嘌呤氧化及脉冲场凝胶电泳法分析了辐照后的细胞存活率、胞内SOD活性及细胞DNA双链断裂水平。研究发现,随着γ辐照吸收剂量的增大,细胞存活率不断下降;SOD活性随剂量的变化无明显的规律;DNA双链断裂水平与细胞存活率密切相关,DNA的释放百分比和断裂水平值随辐照剂量增加而不断增大。结果表明:γ辐照对枯草芽孢杆菌营养体有较高的灭活能力,其损伤效果可能与SOD活性及双链断裂相关。In order to investigate the damage effects of γ-rays at cell and molecular level,Bacillus subtilis vegetative cells were irradiated by 60Co γ-rays at different absorbed doses.The cell survival rate was examined with the standard plate-count method.The intracellular SOD activity was measured by SOD kit through xanthine oxidase method.DNA double-strand breaks were analyzed by pulsed-field gel electrophoresis（PFGE）.The cell survival rate decreases when γ-rays dose increases.A clear relation could not be found between intracellular SOD activity and absorbed dose.The DNA release percentage value and break level value increase obviously with γ-rays dose.Cell survival rate is related to DNA double-strand breaks level.It can be concluded that γ-rays have obviously damage effect on Bacillus subtilis vegetative cell,and the damage effect changes with SOD activity and DSB.

关 键 词：Γ辐照 SOD活性 双链断裂 辐照灭菌 

分 类 号：Q142.6[生物学—生态学] Q691.8[生物学—普通生物学]