毛细管电泳-单链构象多态分析检测胃癌中p16基因突变  

p16 Gene Mutation Detection by Single Strand Conformation Polymorphism with Capillary Electrophoresis in Gastric

作　　者：谢希晖[1,2] 王荣[1,2] 贾正平[1,2] 谢华[1] 张爱梅[3] 

机构地区：[1]兰州军区兰州总医院临床药理基地,甘肃兰州730050 [2]兰州大学药学院,甘肃兰州730000 [3]西北师范大学生命科学院,甘肃兰州730070

出　　处：《生物技术通讯》2011年第6期801-805,共5页Letters in Biotechnology

基　　金：国家自然科学基金(20775089)

摘　　要：目的:建立快速高效检测胃癌患者胃癌组织及癌旁正常组织中p16基因突变的方法。方法:采用PCR扩增p16基因第二外显子易发生突变片段,扩增样品纯化后经95℃变性;以毛细管电泳(CE)分析法结合单链构象多态性(SSCP)对60例胃癌患者p16基因突变情况进行分析。结果:分析结果表明只有3例低分化腺癌患者存在基因突变,测序表明p16基因第二外显子碱基序列AGAC发生碱基A丢失。结论:p16基因突变可能导致胃癌的发生,但不起主导作用;CE-SSCP分析方法具有快速、灵敏、准确的特点,可用于胃癌组织中p16基因的突变分析。Objective： To obtain high performance detection method for detecting p16 gene mutation in gastric cancer tissue and normal tissue.Methods： The part of p16 gene exon 2 which liability mutation was amplified by PCR,then amplified samples were purified and denatured at 95℃.p16 gene mutation in 55 gastric cancer was detected by capillary electrophoresis（CE）-single strand conformation polymorphism（SSCP）.Results： The results revealed that only three poorly differentiated adenocarcinoma patients have gene mutation.Further sequence analysis emonstrated that base sequence AGAC detected base A in p16 gene exon 2.Conclusion： This study showed p16 gene mutation may induce gastrric cancer,but not play an inportant role in neoplastic processes.CE-SSCP has the advantage of fast,sensitive and accurate,which can use in analysis of p16 gene mutation.

关 键 词：P16基因 基因突变 毛细管电泳 单链构象多态性 胃癌 

分 类 号：Q502[生物学—生物化学] Q78