发酵生产龙血竭中龙血素A和B的分析  

Analysis of Loureirins A and B in the Dragon's Blood Produced by Fermentation

作　　者：赵洁[1] 李尚真[1] 宋启示[1] 

机构地区：[1]中国科学院西双版纳热带植物园,昆明650223

出　　处：《天然产物研究与开发》2011年第6期1163-1166,共4页Natural Product Research and Development

基　　金：中国科学院知识创新工程重要方向项目子课题(07ZK171K01);国家863项目子课题(2007AA021504)

摘　　要：以高效液相色谱法,检测血竭诱导菌在不同发酵时间下发酵龙血树干粉和龙血树愈伤组织的产物中龙血素A和龙血素B的含量。干粉发酵产物中,龙血素A的含量大于愈伤组织发酵产物中的含量,并且在第四周时达到最大值0.253 mg/g;龙血素B在第三周时达最大值0.519 mg/g。愈伤组织发酵产物也含有少量的龙血素A和龙血素B。虽然发酵产物中的龙血素B远远高于龙血树根、茎、叶中的含量,但还是低于0.4%的质量标准,因此不能应用于生产。In this study, the contents of loureirins A and B in the fermentation products from the dry powder and the callus of Dracaena cochinchinensis were determined by HPLC with different fermentation time. The results showed that the dry powder produced more loureirin A during fermentation comparing with the callus of Dracaena cochinchinensis, and the content of loureirin A reached a peak of 0.253 mg/g by four-week fermentation. Loureirin B reached a peak of 0. 519 mg/g after three weeks＇ fermentation with the dry powder. Yet, the callus produced a small amount of louroirins A and B during the fermentation process. Although the content of loureirin B obtained by fermentation was much higher than those in the roots, stems, and leaves of Dracaena cochinchinensis, this process can still not be applied in drug production since the produced amount was lower than the quality standard of 0.4%.

关 键 词：龙血树 龙血竭 发酵 高效液相色谱法 龙血素A 龙血素B 

分 类 号：R284.1[医药卫生—中药学]