SRAP和ISSR及两种方法结合在分析黄麻属起源与演化上的比较  

Method Comparison of Using SRAP and ISSR and Combination of Both in Origin and Evolution of Jute

作　　者：陶爱芬[1] 祁建民[1] 粟建光[2] 李爱青[3] 李木兰[1] 方平平[1] 林荔辉[1] 徐建堂[1] 吴建梅[1] 林培清[1] 

机构地区：[1]作物遗传育种与综合利用教育部重点实验室/福建农林大学,福建福州350002 [2]中国农业科学院麻类研究所,湖南长沙410205 [3]安徽省种子管理总站,安徽合肥230001

出　　处：《作物学报》2011年第12期2277-2284,共8页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31000734);国家麻类产业技术体系(CARS-19-E06);南方麻类种质资源平台建设项目(2010N2002)资助

摘　　要：选用96份黄麻属种质资源,比较了SRAP、ISSR及二者结合的方法在黄麻属起源与演化研究上的可行性。结果表明:(1)SRAP方法的多态性条带比率为100%,高于ISSR方法的98.1%,平均每条引物扩增出的多态性条带亦高于ISSR方法。(2)SRAP方法构建的进化树可大致将各类型种质资源区别开来,可较明确、清晰地展现黄麻属的起源与演化趋势。但无法区分开个别种质;ISSR方法构建的进化树将很多圆果黄麻品种聚在一起,无法区别开来,且分支长度短,无法确定进化时间;(3)SRAP与ISSR结合构建的进化树将不同类型的黄麻种质资源有序排列,可清晰地明确其进化趋势及演化关系。比较而言,SRAP与ISSR分子标记结合的方法优于SRAP方法,而SRAP方法又优于ISSR方法,在进行相关研究时,应优先考虑采用SRAP与ISSR分子标记结合的方法。The objective of this study was to compare the feasibility of using SRAP,ISSR and combination of the both in origin and evolution of jute（Corchorus L.）.Ninety-six jute genotypes were tested by these three methods.The results were as follows：（1） The percentage of polymorphic bands of SRAP was 100%,which was higher than that of 98.1% of ISSR,and the polymorphic bands amplified per primer by SRAP were also higher than these of ISSR.（2） On the dendrogram based on SRAP data,jute genotypes were close to be classified.The trend of origin and evolution of jute could be clarified.However,a few genotypes were misled to other taxonomic groups,which affected the accuracy of analysis on origin and evolution of jute.On the dendrogram based on ISSR data,many capsularis cultivars were found to be clustered together and could not be identified clearly,and also the time of origin was dif-ficult to confirm.（3） Based on the dendrogram derived from SRAP and ISSR data,different taxonomic groups of jute were found to be clustered orderly,and the relationship of origin and the trend of evolution within jute genus manifested clearly.（4） The strategy of SRAP and ISSR combination was better than that of SRAP alone,and then followed by ISSR.Hence,the strategy of SRAP and ISSR combination should have priority in studies on the origin and evolution of jute.

关 键 词：黄麻 起源 演化 SRAP ISSR 

分 类 号：S563.4[农业科学—作物学]