下牙槽神经缺失对下颌骨骨缺损骨痂血肿期炎症因子表达影响的实验研究  

The effects of transection of inferior alveolar nerve on inflammatory factors during haematoma phases in mandibular defect healing of denervated rabbits

作　　者：马高旗[1] 李焰[1] 张纲[1] 谭颖徽[1] 

机构地区：[1]第三军医大学新桥医院口腔科,重庆400037

出　　处：《华西口腔医学杂志》2011年第6期640-642,共3页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30973334;30772436);四川省卫生厅基金资助项目(100086)

摘　　要：目的探讨下颌骨骨缺损愈合过程中，下牙槽神经缺失对血肿期骨痂中肿瘤坏死因子-α（TNF-α）、白细胞介素一1（IL-1）和白细胞介素-6（IL-6）表达的影响。方法50只中国大白兔随机分作对照组和实验组，分别建立单纯下颌骨骨缺损和失下牙槽神经下颌骨骨缺损动物模型，于术后1、2、3、5和7d获取骨痂冰冻保存，酶联免疫吸附试验（ELISA）检测骨痂中TNF-α、IL-1和IL-6蛋白表达，逆转录聚合酶链反应（RT-PCR）检测TNF-αmRNA的表达。结果骨缺损后1d，实验组骨痂中TNF-α、IL-1和IL-6均明显低于对照组，分别为对照组的75．1％、68．3％、72．7％（／9〈0．05）；骨缺损后2d，实验组IL-1明显低于对照组，为对照组的75．5％，而TNF-α则高于对照组；骨缺损后3-7d，TNF-α、IL-ISNIL-6均呈下降趋势，2组之间无明显差异。在各时间点，实验组TNF-α mRNA表达皆低于对照组。结论在下颌骨骨缺损愈合初期，下牙槽神经缺失使神经介质分泌减少，导致局部炎症反应降低或延迟，对骨痂的正常愈合产生负面影响。Objective To study the effects of transection of inferior alveolar nerve （IAN） on the healing of mandibular fracture by investigating the changes of inflammatory factors expression, including tumor necrosis factor-α（TNF-α）, interleukin-1 （IL-1） and interleukin-6（IL-6） in the denervated rabbits＇ bony callus. Methods 50 Chinese rabbits were randomly divided into the experimental group and control group, the experimental group underwent mandibular fracture and transection of IAN and the control group underwent mandibular fracture only. The bony callus were col- lected at 1, 2, 3, 5 and 7 d after operation, respectively. The expression of TNF-α, IL-1 and IL-6 in callus was detected by enzyme linked immunosorbent assay （ELISA）, and the mRNA of TNF-α was detected by reverse transcription-polymerase chain reaction（RT-PCR）. Results In the experimental group, at the 1st day after operation, the expression of TNF-α（75.1%）, IL-1 （68.3%） and IL-6（72.7%） was lower than that in control group（P〈0.05）. At the 2nd day, the expression of IL-1 （75.5%） was still lower than that in control group, but the expression of TNF-α was higher than that in control group. In the next observation points, all inflammatory factors decreased, and there was no difference between experimental and control groups. In RT-PCR, the detection of TNF-α mRNA in the experimental group was lower than that in control group. Conclusion In the haematoma of mandibular fracture healing, transection of IAN causes neuro-humor decrease, depression and delay of inflammatory reaction. It is unfavorable to fracture healing.

关 键 词：下颌骨骨缺损 下牙槽神经 肿瘤坏死因子-α 白细胞介素-1 白细胞介素-6 

分 类 号：R782.4[医药卫生—口腔医学]