用微生物法进行手性化合物立体选择性拆分的初步研究  

PRELIMINARY INVESTIGATION OF ENANTIOSELECTIVE HYDROLYSIS OF CHIRAL ESTER FROM MICROORGANISMS

作　　者：张金红[1] 姚传义[1] 俞耀庭[1] 何朝辉[2] 杨先芹[2] 杨文博[2] 

机构地区：[1]南开大学分子生物学研究所,生物活性材料研究实验室,天津300071 [2]南开大学微生物学系,300071

出　　处：《南开大学学报（自然科学版）》1999年第4期39-41,46,共4页Acta Scientiarum Naturalium Universitatis Nankaiensis

摘　　要：用从生产手性药物工厂附近的土壤中筛选出的17株细菌,对布洛芬氯乙酯拆分进行了初步研究,实验结果显示,在LB培养基中,17株细菌分别在12～36h达到对数生长期;它们都有不同程度的水解手性化合物的能力,其中有的还具有较强的立体选择性;17株细菌中只有7株的脂肪酶活性较高,但脂肪酶活性与转化能力及立体选择性不一致.Microorganisms were screened for the production of chiral medicinal product. The bacteria were isolated from soil samples in the vicinity of the factory producing the chiral drug. We obtained 17 strains in the solid and liquid medium containing Tween 80. The activity of lipase was determined and the ratio of ( S+,R- )profen was tested. Experimental results show that these bacteria have different logarithmic growing phase which range from 12 to 36 hours. When the esterified substrate was used, ( S+,R- )profen enantioselective products were produced. Some strains of the bacteria can produce R - profen, while others can produce S + profen. Among the 17 screened strains of bacteria we found 7 strains of high lipase activity and the range of lipase activity is from 136 to 800 μmol NPP/mL at 5min. The lipase is found to be extracellular.

关 键 词：手性化合物 拆分 微生物法 立体选择性 手性药物 

分 类 号：R914.4[医药卫生—药物化学] O621.2[医药卫生—药学]