SDF-1α和CXCR4在低氧缺糖海马脑片中的表达变化  

Expressional changes of SDF-1α and CXCR4 proteins in oxygen-glucose deprivation hippocampal organotypic slice culture of rat

作　　者：王玉兰[1] 徐红[1] 李爱娜[1] 

机构地区：[1]徐州医学院人体解剖教研室,徐州221002

出　　处：《安徽医科大学学报》2011年第12期1238-1241,共4页Acta Universitatis Medicinalis Anhui

基　　金：江苏省高校自然科学研究计划项目(编号:08KJB310013);徐州医学院院长专项人才基金(编号:08KJZ03)

摘　　要：目的研究海马脑片低氧缺糖损伤后SDF-1α和CX-CR4蛋白的表达变化情况。方法运用脑片培养技术建立低氧缺糖海马脑片模型,用免疫组化法和Western blot法检测SDF-1α和CXCR4的表达变化。结果低氧缺糖损伤前后海马脑片中均可见SDF-1α和CXCR4表达的阳性细胞,其胞膜和胞质呈棕黄色着色,其中部分轴丘可见CXCR4阳性蛋白表达。Western blot发现,损伤后在分子量为11 ku和43ku处分别检测到SDF-1α、CXCR4阳性条带;与正常对照组相比,OGD组SDF-1α和CXCR4表达均增加,其中SDF-1α表达增加差异有统计学意义(P<0.01)。结论海马脑片缺氧缺糖损伤后SDF-1α蛋白表达增加,提示SDF-1α可能与海马脑片低氧缺糖损伤密切相关。Objective To investigate expressional changes of SDF-1α and CXCR4 proteins in oxygen-glucose deprivation（OGD） hippocampal slice. Methods Western blot and immunohistochemistry analyses were used to detect SDF-1α and CXCR4 proteins expression in hippocampal slice of HOTC group and OGD group. Results Immunohistochemistry showed that the SDF-1α and CXCR4 proteins were expressed in hippocampal slice both HOTC group and OGD group, its hyalomitome was stained, and the CXCR4 protein expression was detected in the axon hillock of pyramidal cells. Western blot results showed that the expressions of SDF-1α and CXCR4 proteins increased after OGD, and the expression of SDF-1α proteins had significantly increased （P 〈 0. 01 ）. Conclusion The expressional changes of SDF-1α and CXCR4 proteins indicate that SDF-1α is associated with the OGD of hippocampal slice.

关 键 词：SDF-1Α CXCR4 低氧缺糖 脑片培养 大鼠 

分 类 号：R743.34[医药卫生—神经病学与精神病学]