大鼠C6胶质瘤放射治疗前后^18F—FDG摄取水平变化与多种生物学指标的相关性研究  

The correlations between ^18 F-FDG uptake and a variety of tumor biological indicators in C6 glioma rats with radiotherapy

作　　者：李伟龙[1] 邢喜玲[1] 蔡莉[1] 刘桂超[1] 李彦生[1] 高硕[1] 

机构地区：[1]天津医科大学总医院PET／CT中心,300052

出　　处：《中华核医学杂志》2011年第6期368-372,共5页Chinese Journal of Nuclear Medicine

摘　　要：目的探讨大鼠C6胶质瘤放疗前后^18F-FDG摄取水平变化及其与肿瘤细胞密度、Glut、细胞增殖及血管生成的关系。方法30只雄性荷C6胶质瘤SD大鼠按随机数字表法分为A、B和C3组，每组10只，均接种肿瘤细胞。2周后，A组行^18F-FDG PET／CT显像，B和C组分别于放疗后48h、1周行^18F-FDG PET／CT显像，计算肿瘤与对侧脊柱旁肌肉的SUVmax比值（TIM），观察放疗前后肿瘤TIM的变化。显像后处死大鼠，取出肿瘤组织，观察组织病理变化，并计数肿瘤细胞密度，免疫组织化学法测定肿瘤Ki67标记指数（Ki67 LI）及微血管密度（MVD），Western blot法检测Glut-1及VEGF的表达，采用单因素方差分析比较放疗前后T／M及各指标的变化，双变量相关分析评价T／M变化与各生物学指标间的相关性。结果（1）A、B、C3组T／M、肿瘤细胞密度、Ki67LI、MVD、Glut-1及VEGF水平总体比较组间差异均有统计学意义（F值依次为6．77，60．66，104．56，95．49，9．13和24．48，P均〈0．05）。两两比较示，B组T／M（9．23±4．56）、肿瘤细胞密度（672．70±92．98）及Ki67 LI（18．56±2．26）与A组（10．86±3．31，730．50±78．93，20．02±2．14）差异无统计学意义（P均〉0．05），C组（5．16±2．52，355．60±72．62，7．81±1．76）与A、B2组相比下降明显，差异均有统计学意义（P均〈0．05）；B组MVD（19．50±1．96）及Glut-1水平（0．20±0．09）较A组（17．90±2．02，0．15±0．04）差异无统计学意义（P均〉0．05），C组该两指标（8．40±1．84，0．07±0．06）显著下降，且与A、B2组间差异均有统计学意义（P均〈0．05）。B组肿瘤VEGF表达（0．42±0．13）与A组、C组（分别为0．17±0．04，0．16±0．09）相比显著增高，差异均有统计学意义（P均〈0．05）。（2）A、B组间T／M变化与肿瘤细胞密度变化呈明显正相关（r=0．81，P〈0．05）；A、C组间T／MObjective To study the relationship between is F-FDG uptake and tumor cell density, glucose transporter expression, cellular proliferation and angiogenesis before and after radiotherapy in C6 glioma rats. Methods Thirty C6 glioma-bearing male SD rats were randomly divided into three groups： A, B and C （10 rats in each group）. Two weeks later, lSF-FDG PET/CT was performed in group A. In groups B and C, ^18F-FDG PET/CT was performed at 48 h and 1 week after radiotherapy, respectively. The ratio of SUVmax of tumor to muscle （T/M） was calculated. HE staining, immunohistochemical staining and Western blot were used to measure tumor cell density, Ki67 labeling index （ LI）, microvessel density （ MVD）, Glut-1 and VEGF expression quantitatively. The one-way analysis of variance and bivariate correlation analysis were used to compare the changes of each indicator and evaluate the correlation between T/M and bio logical indicators, respectively. Results Significant differences of T/M, tumor cell density, Ki67 LI, MVD, Glut-1 and VEGF among groups A, B and C were observed （F = 6.77, 60.66, 104.56, 95.49, 9. 13, 24.48, respectively, all P 〈0.05）. Least significant difference （LSD） test showed that there was no significant difference between group A and B in T/M, tumor cell density and Ki67 LI （ 10.86 ± 3.31, 730. 50 ±78.93,20.02 ±2.14 vs 9.23 ±4.56, 672.70 ±92.98, 18.56 ±2.26）. However, the indicators of group C （5.16 ±2.52,355.60 ±72.62,7.81 ±1.76） were significantly decreased compared with those of groups A and B （ all P 〈 0.05 ）. MVD and Glut-1 expression of group B increased slightly compared with those of group A （ 19.50 ±1.96, 0. 20 ±0.09 vs 17.90 ±2.02,0.15 ±0.04）, but the difference was not statistically significant. Nevertheless, the two indicators were significantly decreased in group C （ 8.40 ±1.84 and 0. 07 ±0. 06, P 〈 0. 05 ）. VEGF expression in group B （0.42 ±0. 13 ） was significantly higher than that in groups A and C （0.17±0.04 andO. 16

关 键 词：神经胶质瘤 肿瘤细胞 培养的 放射疗法 体层摄影术 发射型计算机 体层摄影术 X线计算机 脱氧葡萄糖 大鼠 

分 类 号：R739.4[医药卫生—肿瘤] R817.5[医药卫生—临床医学]