咽喉鳞状细胞癌患者外周血T淋巴细胞亚群和NK细胞活性研究  

Analysis of serum T-lymphocyte subsets and NK cell activity in patients with squamous cell carcinoma of pharynx and larynx

作　　者：陈万军[1,2] 王兴武[1,2] 陶荣杰[1,2] 

机构地区：[1]山东省肿瘤医院头颈科 [2]山东省医学科学院,济南250117

出　　处：《临床耳鼻咽喉头颈外科杂志》2011年第24期1105-1107,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：山东省优秀中青年科学家科研奖励基金(No:2008BS03034);山东省医药卫生科研项目(No:2005HW136)

摘　　要：目的:研究咽喉癌患者外周血T淋巴细胞亚群分布和NK细胞活性及其意义。方法:应用流式细胞仪检测123例咽喉癌患者及36例正常志愿者空腹外周静脉血淋巴细胞亚群和NK细胞活性。结果:咽喉癌患者外周血总T淋巴细胞百分比低于正常人(P<0.05),辅助T淋巴细胞百分比略低于正常人(P>0.05),抑制T淋巴细胞百分比高于正常人(P<0.01),辅助/抑制T淋巴细胞比值低于正常人(P<0.05)。外周血总B淋巴细胞咽喉癌患者略低于正常人(P>0.05),活化T细胞正常人略低于咽喉癌患者(P>0.05),NK细胞活性比较咽喉癌患者低于正常人(P<0.01)。Ⅰ～Ⅱ期外周血总T淋巴细胞略低于Ⅲ～Ⅳ期患者(P>0.05),Ⅰ～Ⅱ期辅助T淋巴细胞含量略高于Ⅲ～Ⅳ期患者(P>0.05),Ⅰ～Ⅱ期抑制T淋巴细胞比值低于Ⅲ～Ⅳ期患者(P<0.01),Ⅰ～Ⅱ期辅助/抑制T淋巴细胞比值高于Ⅲ～Ⅳ期患者(P<0.01)。Ⅰ～Ⅱ期外周血总B淋巴细胞相对值高于Ⅲ～Ⅳ期患者(P<0.05),活化T细胞在Ⅰ～Ⅱ期和Ⅲ～Ⅳ期患者变化不大(P>0.05);Ⅰ～Ⅱ期外周血NK细胞比值略高于Ⅲ～Ⅳ期患者(P>0.05)。结论:咽喉癌患者存在免疫功能紊乱或低下,随病程进展,不仅细胞免疫功能低下,体液免疫功能也逐渐降低,表明免疫功能紊乱在咽喉癌的发病及病程进展中可能有一定作用。应用流式细胞仪检测外周静脉血淋巴细胞亚群及NK细胞活性的变化来个体化评价咽喉癌患者免疫功能,简单易行,值得推广。Objective：To investigate the alteration of T-lymphocyte subsets and NK activity in patients with squamous cell carcinoma of pharynx and larynx.Method：T-lymphocyte subsets and NK activity were determined by flow cytometry in 123 patients with squamous cell carcinoma of pharynx and larynx.Blood samples of 36 non-tumor patients were used as control.Result：The total T lymphocytes were lower in patients with squamous cell carcinoma of pharynx and larynx than control group significantly（P0.05）.The levels of helper lymphocyte subsets were little lower than those in control group（P0.05）.On the other hand,the levels of suppressor lymphocytes in patients were higher than those in the control group（P0.05）.Therefore,the CD4/CD8 ratios in patients were lower than those of the control group statistically（P0.05）.There was no statistical difference in activated T lymphocytes and total B lymphocytes（P0.05）,but NK activity in patients were lower than those in control group significantly（P0.01）.There was no statistical difference in total T lymphocytes between stage Ⅰ-Ⅱ and stage Ⅲ-Ⅳ（P0.05）.The levels of helper lymphocyte subsets in stage Ⅰ-Ⅱ patients were little higher than in stage Ⅲ-Ⅳ patients（P0.05）,but the levels of suppressor lymphocytes in stage Ⅰ-Ⅱ patients were lower than in stage Ⅲ-Ⅳ patients（P0.01）.The CD4/CD8 ratios in stage Ⅰ-Ⅱ patients were significantly higher than in stage Ⅲ-Ⅳ patients（P0.01）.The levels of total B lymphocytes in stage Ⅰ-Ⅱ patients were significantly higher than in stage Ⅲ-Ⅳ patients（P0.05）.The activated T lymphocytes and NK activity did not changed statistically（P0.05）.Conclusion：The immune function in patients with squamous cell carcinoma of pharynx and larynx is disordered and lower.With advanced stage disease,not only the cellular immune function in patients decrease gradually,but also the humoral immunity is lower.Analyzing T-lymphocyte subsets and NK activity determined by flow cytometry would be eas

关 键 词：咽肿瘤 喉肿瘤 淋巴细胞亚群 NK细胞 

分 类 号：R739.6[医药卫生—肿瘤]