培养条件对赭曲霉菌球形成和坎利酮11α羟基化的影响  

Effects of culture conditions on pelletes growth form of Aspergillus ochraceus for canrenone 11αhydroxylation

作　　者：谢进霞[1] 别松涛[1] 刘逸寒[1] 徐星 李建勋 刘晓[1] 路福平[1] 

机构地区：[1]工业发酵微生物教育部重点实验室工业酶国家工程实验室天津市工业微生物重点实验室天津科技大学生物工程学院,天津300457

出　　处：《工业微生物》2011年第6期6-10,共5页Industrial Microbiology

基　　金：天津市科技计划项目(编号:09ZCKFSH00600)

摘　　要：菌丝粗壮、分布均匀的菌球可提高赭曲霉11α羟基化的效率。控制赭曲霉菌球直径的平均大小可以通过选择不同的培养温度、装液量、以及转速等因素而达到。通过正交分析，确定培养温度为30℃，按孢子悬液浓度为10。个／mL接种，装液量为50mL／250mL三角瓶，摇床转速为200r／min时，菌球达到最佳直径2～3rnrn之间的百分比最大。在此条件下，以10g/L的坎利酮投料，最终转化率高达91．26％。The efficiency of 11α hydroxylation could be improved by Aspergillus ochraceu pellets with thick hyphae and uniform distribution. The experiment indicated that the mean size of pellets could be controlled by different culture temperature, inoculation volume, rotation speed and other factors. Through the orthogonal analysis, when the culture temperature was 30 ℃, the concentration of spore suspension was inoculated with 108 spores/mL, medium volume 50 mL/250 mL flask, shaker speed 200 r/min, the maximum rate of the pellet diameter from 2 mm to 3 mm was obtained. Under these conditions by adding 10 g/L of canrenone, the final conversion rate reached as high as 91.26 %.

关 键 词：赭曲霉 菌球形态 坎利酮11α羟基化 转化率 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]