纳米二氧化硅对秀丽线虫的毒性作用研究  

Toxicity of SiO_2 Nanoparticles to Caenorhabditis Elegans

作　　者：孔璐[1] 张婷[1] 王大勇[2] 唐萌[1] 浦跃朴[1] 

机构地区：[1]东南大学公共卫生学院环境医学工程教育部重点实验室,南京210009 [2]东南大学医学院,南京210009

出　　处：《生态毒理学报》2011年第6期655-660,共6页Asian Journal of Ecotoxicology

基　　金：国家重大科学研究计划(2011CB933404);国家自然科学基金(30972504)

摘　　要：为探讨二氧化硅纳米颗粒(SiNP)的毒性作用。以微米SiO2和空白做对照,将尺寸为20和60nm的SiNP分别以0.2,1.0和5.0g·L-13个浓度对模式生物秀丽隐杆线虫进行染毒。通过第一代和第二代线虫体长、身体弯曲频率、头部摆动频率以及第一代线虫后代数目、世代时间等发育、运动和生殖的相关评价指标,对SiNP毒性作用进行综合评价。结果显示,与空白对照组和微米组比较,第一代和第二代线虫在5.0g·L-120和60nm SiO2剂量组均出现发育和运动等异常(p<0.01);暴露在60nmSiO2时,3个剂量组的秀丽隐杆线虫均出现生殖指标的异常(p<0.01),而20nm SiO2的各剂量组秀丽隐杆线虫的生殖指标均未出现有统计学意义的改变。在5g·L-1剂量组,20和60nmSiO2及微米SiO23组中两两比较差异有统计学意义(p<0.01)。结果说明,纳米尺寸和暴露浓度可影响纳米SiO2对秀丽隐杆线虫的运动、生殖和发育等多系统功能。此研究工作可为制定SiNP的接触限值标准提供参考。The aim of this study was to investigate the toxicity of SiO2 nanoparticles by using a model organism C. ele- gouts. C. elegc^s were treated with 0.2, 1.0 and 5.0 g＂ L 1 of 20 nln or 60 nm SiO2 nanoparticles, and the control group were treated with 5.0 g＂ L ~ of SiO2 microparticle or 0.9% saline. The toxicity was detected using a battery of bio- tests. The indicator included body size, body bends, head thrashes of first-and second-generation C. elegans, brood type and generation time of fwst-generation C. elegans. Compared with control and microparticle groups, abnormities in de- velopment and locomotion were induced in the first-generation and second-generation C. elegans which were exposed to 5.0 g＂ L-~20 and 60 nm SiO2 nanoparticles （p 〈0.01）. Abnormities of reproductive indicators were observed in brood type and generation time when exposure dose of 60 nm SiO2 nanoparticles exceeded 0.2 g. L-l（p 〈 0.01）. 20 nm SiO2 nanoparticles had no significant toxicity on the reproduction of C. elegans. Statistical analysis showed a significant difference between the toxicities of SiO2 nano- and micronparticle treatments at 5 g, L- ~ SiO2 nanoparticle solution. The results showed that the scale and concentrations of SiO2 can affect its toxicity on development, locomotion behavior and reproduction of C. elegans. The above results could provide a reference for determining the exposure limit standardfor SiO2 nanoparticles.

关 键 词：纳米二氧化硅 秀丽隐杆线虫 纳米毒理 

分 类 号：X171.5[环境科学与工程—环境科学]