急性单核细胞白血病免疫表型研究  

Immunophenotypic Study on the Acute Monocytic Leukemia

作　　者：姚锡珍[1] 李建勇[1] 夏学鸣[1] 阮长耿[1] 耿美菊[2] 王爱青[2] 陈黎[2] 朱明清[2] 

机构地区：[1]苏州医学院附属第一医院血液科,江苏省血液研究所苏州215006 [2]苏州医学院核医学生物技术重点实验室

出　　处：《苏州医学院学报》1999年第12期1283-1284,共2页Acta Academiae Medicinae Suzhou

摘　　要：目的　探讨急性单核细胞白血病（Ｍ５）的免疫表型特征及其诊断价值。方法　采用一组系列相关单克隆抗体和流式细胞术及间接免疫荧光技术对６０ 例ＦＡＢ分型拟诊为Ｍ５ 的患者进行免疫表型分析。结果　４ 例诊断为急性巨核细胞白血病，５ 例为急性淋巴细胞白血病；５１ 例最后诊断为Ｍ５ 的患者中，免疫分型显示８％ 裸型，４９％ 表达ＣＤ３４ ，髓系标志以ＣＤ１３ 表达最高，其次为ＣＤ３３ 和ＣＤ１４，分别有２４ ％ 和１８％ 的患者表达Ｔ 系和Ｂ系相关抗原。Objective To explore the immunophenotypic feature of acute monocytic leukemia(M5) and its diagnostic significance. Methods A panel of lineage associated monoclonal antibodies and flow cytometric immunofluorescence technique were used to immunophenotype 60 cases with FAB diagnosed M5. Results Combining with immunophenotype, 4 and 5 cases were diagnosed as acute megakaryocytic leukemia and acute lymphoblastic leukemia respectively, and 51 cases were finally diagnosed as M5. Of the 51 cases with M5,immunophenotype showed that 8% was nude,49% expressed CD34,as for the myeloid markers, CD13 were more frequently expressed,then CD33 and CD14. T and B lineage associated antigens were expressed in 24% and 18%,respectively. Conclution Immunophenotyping was important for the diagnosis of acute monocytic leukemia.

关 键 词：急性 单核细胞 白血病 免疫表型 流式细胞术 

分 类 号：R733.710.4[医药卫生—肿瘤]