低氧条件下成骨细胞的增殖与分化  

Effects of hypoxia on proliferation and differentiation of rat osteoblasts

作　　者：高文魁[1] 王德元[1] 李智钢[1] 闫自强[1] 白峰[1] 王伟[1] 

机构地区：[1]解放军第四医院骨科,青海省西宁市810007

出　　处：《中国组织工程研究与临床康复》2011年第46期8591-8594,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：青海省科学技术厅科技创新能力促进计划项目(2010.Z.744);课题名称:低氧环境在骨质疏松发生发展过程中的作用机制研究~~

摘　　要：背景:低氧可通过多种途径作用于成骨细胞影响骨代谢,对骨生成、骨愈合等产生负面影响。目的:观察低氧对体外培养大鼠成骨细胞增殖、分化的影响,并探讨其分子机制。方法:分离培养新生Wistar大鼠颅盖骨成骨细胞,取第2代细胞分别在常氧(体积分数20%O2)与低氧(体积分数3%O2)条件下培养。结果与结论:低氧组成骨细胞增殖、碱性磷酸酶活性、骨钙素水平及茜素红结节形成数量均明显低于常氧组(P<0.05或P<0.01),说明缺氧条件对成骨细胞的增殖、分化及功能有抑制作用;低氧组骨形成发生蛋白2及Runx2表达低于常氧组(P<0.05或P<0.01),说明低氧条件下大鼠成骨细胞Runx2、骨形成发生蛋白2的表达受抑制。结果表明低氧可通过抑制大鼠成骨细胞的Runx2、骨形成发生蛋白2的表达进一步抑制成骨细胞的增殖与分化。BACKGROUND：Hypoxia influences bone metabolism by many means and exhibits negative effects on bone generation and bone healing.OBJECTIVE：To explore the effects of hypoxia on proliferation and differentiation of rat osteoblasts and investigate the underlying mechanisms.METHODS：Primary rat osteoblasts were isolated from excised calvarial bones of neonatal mice and cultured in vitro.The second passage of osteoblasts were cultured under hypoxic（3% O2） and normoxic（20% O2） conditions.RESULTS AND CONCLUSION：The proliferation levels,alkaline phosphatase activities,osteocalcin contents and the number of calcium nodules in the hypoxic group were significantly lower compared with the normoxic group（P〈0.05 or P〈0.01） .It means that hypoxia inhibits the proliferation,differentiation and functions of rat osteoblasts.The mRNA expression of bone morphogenetic protein-2 and Runx2 in osteoblasts cultured in 3% O2 was lower than that cultured in 20% O2（P〈0.05 or P〈0.01） .It means that the mRNA expression of Runx2 and bone morphogenetic protein-2 was decreased in hypoxic conditions.These findings suggest that hypoxia can inhibit the proliferation and differentiation of rat osteoblasts through inhibiting Runx2 and BMP-2 mRNA expression in vitro.

关 键 词：低氧 成骨细胞 增殖 分化 RUNX2 骨形成发生蛋白2 

分 类 号：R318[医药卫生—生物医学工程]