nm 23基因参与肿瘤转移抑制机制的研究  

The Study of the Mechanism that nm23 Involved in the Course of Cancer Metastasis Suppressing

作　　者：崔宏[1] 何三光[1] 刘永锋[1] 崔桂英[2] 郝君[3] 李金鸣[4] 

机构地区：[1]中国医科大学第一临床学院普外科,沈阳110001 [2]中国医科大学基础医学院生理学教研室 [3]抚顺矿务局总医院普外科 [4]中国医科大学基础医学院药理学教研室

出　　处：《中国医科大学学报》1999年第6期428-430,共3页Journal of China Medical University

摘　　要：目的：探讨ｎｍ２３基因参与肿瘤转移抑制的机制。方法：采用膜片钳制技术全细胞记录方式，对具有不同ｎｍ２３基因表达的不同转移潜能的人胰腺癌ＪＦ３０５单克隆细胞亚系中钙电流特征进行观察。结果：人胰腺癌ＪＦ３０５单克隆细胞亚系中的钙电流以钙释放激活的钙内流方式为主。人胰腺癌ＪＦ３０５Ａ、ＪＦ３０５Ｃ细胞钙电流的大小有显著差异（Ｐ＜０．０１）。结论：钙离子参与了ｎｍ２３基因在人胰腺癌ＪＦ３０５细胞亚系的转移抑制机制。Objective:Try to find the mechanism that nm 23 involved in the course of cancer metastasis suppressing. Methods:Using patch clamp technique with whole cell mode to record the calcium current in human pancreatic cancer JF 305 cell sublines with different metastatic potential and different nm 23 expression. Results:For the first time recorded the calcium current in human pancreatic cancer JF 305 cell sublines, the current was mainly calcium release activated kind; the calcium current in human pancreatic cancer cell sublines had a significance variance. Using patch clamp technique with whole cell mode to record the calcium current in human pancreatic cancer JF 305 cell sublines with different metastatic potential and different nm 23 expression. Results:For the first time recorded the calcium current in human pancreatic cancer JF 305 cell sublines, the current was mainly calcium release activated kind; the calcium current in human pancreatic cancer cell sublines had a significance variance. For the first time recorded the calcium current in human pancreatic cancer JF 305 cell sublines, the current was mainly calcium release activated kind; the calcium current in human pancreatic cancer cell sublines had a significance variance. Conclusion:Ca 2+ ion involved in the mechanism of metastasis suppress caused by nm23 gene in human pancreatic cancer cell sublines.

关 键 词：NM23基因 胰腺肿瘤 肿瘤转移 抑癌基因 

分 类 号：R735.902[医药卫生—肿瘤] R73－37[医药卫生—临床医学]