反复快速皮肤扩张可促进皮瓣表皮生长因子及增殖细胞核抗原的表达  

Repeated rapid skin expansion promotes the expression of epidermal growth factor and proliferating cell nuclear antigen

作　　者：任贵云[1] 王首军 董福生[1] 于美清[1] 郝福良[1] 张旭东[1] 

机构地区：[1]河北医科大学口腔医学院口腔颌面外科河北省口腔医学重点实验室,河北省石家庄市050017 [2]河北省抚宁县人民医院,河北省秦皇岛市066300

出　　处：《中国组织工程研究与临床康复》2011年第46期8643-8647,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：河北省科技支撑计划资助项目(08276101D-59)~~

摘　　要：背景:针对目前临床常用的常规扩张法与快速扩张法的不足,提出了反复快速皮肤扩张法。目的:观察反复快速皮肤扩张对皮瓣表皮生长因子、增殖细胞核抗原表达的影响。方法:雄性新西兰大白兔随机分为3组:反复快速扩张组、快速扩张组、常规扩张组。结果与结论:扩张维持第2,3周,反复快速扩张组皮瓣表皮生长因子表达显著高于快速扩张组、常规扩张组(P<0.05)。除扩张维持第3周外,其余各时间段,反复快速扩张组成纤维细胞增殖细胞核抗原染色阳性率均显著高于其他两组(P<0.05)。扩张维持第4周,反复快速扩张组表皮细胞增殖细胞核抗原染色阳性率显著高于其他组(P<0.05)。提示反复快速皮肤扩张可能通过促进组织细胞合成与分泌皮瓣表皮生长因子,促进了扩张皮肤中成纤维细胞及表皮细胞的增殖。BACKGROUND：Repeated rapid skin expansion has been proposed because of deficiency in conventional expansion and rapid expansion currently used in the clinic.OBJECTIVE：To investigate the effect of the repeated rapid skin expansion on the expression of epidermal growth factor（EGF） and proliferating cell nuclear antigen（PCNA） .METHODS：Forty-eight male rabbits were randomly divided into three groups：repeated rapid expansion,rapid expansion,conventional expansion（16 rabbits per group） .The samples were collected at different phases and were fixed in 10% formaldehyde solution.The expression of EGF and PCNA was observed with immunohistochemistry.RESULTS AND CONCLUSION：At the 2nd and 3rd weeks,EGF expression was significantly greater in the repeated rapid expansion group than in the rapid expansion group and conventional expansion group（P〈0.05） .PCNA positive staining was significantly higher in the repeated rapid expansion group than in the other two groups at each time stage except for the 3rd week（P〈0.05） .These findings suggest that repeated rapid skin expansion can promote the proliferation of fibroblasts and epidermal cells by raising the content of EGF in the expanded skin.

关 键 词：反复快速皮肤扩张 皮瓣表皮生长因子 增殖细胞核抗原 免疫组织化学 兔 

分 类 号：R318[医药卫生—生物医学工程]