体外培养慢性粒细胞白血病患者骨髓间充质干细胞的生物学特性  

Biological characteristics of chronic myelogenous leukemia-derived Flk1+CD31-CD34-bone marrow mesenchemal stem cells

作　　者：黄栋[1] 李巍[1] 刘羽[1] 朱希山[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院(北京大学第九临床医学院)神经内科,北京市100038

出　　处：《中国组织工程研究与临床康复》2011年第45期8376-8380,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：首都医科大学附属北京世纪坛医院朱希山院级科研基金~~

摘　　要：背景:课题组从胎儿骨髓间充质干细胞的培养体系中鉴定出一类贴壁细胞,已证实此类细胞在单细胞水平可以向造血及内皮细胞分化。目的:检测骨髓间充质干细胞的生物学特性,为慢性粒细胞白血病的治疗提供相关的依据。方法:以慢性粒细胞白血病患者骨髓为研究对象,体外培养并扩增原始间充质干细胞,检测其BCR/ABL融合基因的表达、免疫学特性和生长曲线,使用RT-PCR和FISH的方法检测其BCR/ABL融合基因的表达情况。结果与结论:慢性粒细胞白血病患者骨髓来源的间充质干细胞呈成纤维样生长,大部分细胞处于G0/G1期,并且高表达Flk1,CD13,CD29,CD44,用RT-PCR和FISH的方法能够检测出BCR/ABL融合基因的表达。提示慢性粒细胞白血病的白血病基因转化可能发生在比造血干细胞更高的骨髓间充质干细胞水平上,对慢性粒细胞白血病的疾病起源及干细胞移植治疗有重要的意义。BACKGROUND：We identified a kind of adherent cells from the fetal bone marrow mesenchymal stem cell culture system,which have been confirmed that they can differentiate into hematopoietic and endothelial cells at the single cell level.OBJECTIVE：To detect the biological characteristics of bone marrow mesenchymal stem cells so as to provide the direct evidence for treatment of chronic myelogenous leukemia.METHODS：We isolated and cultured Flk1＋CD31-CD34-cells from chronic myelogenous leukemia patients.Firstly,we study the growth pattern and fluorescence-activated cell sorter of the expanded cells.The expression of the BCR/ABL fusion gene was assayed by nested RT-PCR and FISH.RESULTS AND CONCLUSION：Bone marrow mesenchymal stem cells from chronic myelogenous leukemia patients gave rise to a population of adherent cells with a fibroblast like morphology and had high expansion potential.Cell cycle analysis revealed most of these cells were in the G0/G1 phases.We also showed that more than 90% of these cells were positive stained by FITC labeled Flk1,CD13,CD29,CD44,and negative stained by FITC labeled CD31,CD34,CD45.We could identify the expression of BCR/ABL fusion gene by nested RT-PCR and FISH.These findings provide the direct evidence that rearrangement of BCR/ABL gene might happen at least,but not restrict to the level of hemangioblastic progenitor cells,which are responsible for BCR/ABL fusion gene seen in both hematopoietic cells and endothelial cells.

关 键 词：慢性粒细胞白血病 骨髓间充质干细胞 血液血管干细胞 分化 生物学特性 

分 类 号：R394.2[医药卫生—医学遗传学]