人脐带源间充质干细胞体内外移植的免疫学特征  

Immunological characteristics of human umbilical cord derived mesenchymal stem cells transplanted in vivo and vitro

作　　者：野向阳[1] 徐岩[1] 毕薇薇[1] 郭立斌[1] 李相军[2] 刘建国[3] 

机构地区：[1]吉林省四平市中心医院骨科,吉林省四平市136000 [2]吉林大学药学院病理教研室,吉林省长春市130021 [3]吉林大学第一医院骨关节科,吉林省长春市130021

出　　处：《中国组织工程研究与临床康复》2011年第45期8408-8412,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:部分体外实验证实人脐带源间充质干细胞具有同种异体的免疫耐受特性,然而对其体内研究尚处于异种异体之间,且结果及机制缺少统一性。目的:培养人脐带源间充质干细胞,通过体内外试验分析其移植免疫学特性。方法:应用组织块贴壁法提取人脐带源间充质干细胞,通过体外淋巴细胞混合反应检测其同种异体免疫耐受作用;应用流式细胞仪检测体内行人脐带源间充质干细胞移植前后患者血液中CD4、CD8浓度及比值变化,并通过ELISA方法对比体外淋巴细胞混合培养前后血液、培养上清及体内移植前后患者血液中白细胞介素2,10、γ-干扰素的浓度变化。结果与结论:体内外实验中,混合淋巴细胞反应前后血浆及细胞上清中的细胞因子浓度变化呈相似趋势:白细胞介素10较反应前上升(P<0.05),γ-干扰素较反应前下降(P<0.05)。人脐带源间充质干细胞移植后患者T细胞亚群中CD4+/CD8+比值较移植前轻度下降。说明人脐带源间充质干细胞具有同种异体免疫耐受及免疫调节作用。BACKGROUND：Human umbilical cord derived mesenchymal stem cells（HUC-MSCs） have been confirmed to have the characteristics of immunologic tolerance in vitro,but there are no relevant allogenic research in vivo.The results and mechanism are lack of consistency.OBJECTIVE：To cultivate HUC-MSCs and research the immunological characteristics of implanted HUC-MSCs in vivo and in vitro.METHODS：HUC-MSCs were obtained by using tissue adhesion method.Mixed-leukocyte reaction（MLR） was performed to evaluate immunologic tolerance in vitro,flow cytometry applied to detect the changes of CD4 and CD8 expression as well as ratio of CD4 to CD8,and ELISA used to detect interleukin 2（IL-2）,IL-10,γ-interferon（IFN-γ） levels.RESULTS AND CONCLUSION：MLR results showed a similar trend in the concentration changes of cytokines：the level of IL-10 slightly increased after MLR reaction（P 0.05）,while the level of IFN-γ decreased（P 0.05）.The ratio of CD4＋/CD8＋ in T cell subsets was decreased to a slight extent after cell transplantation,indicating that HUC-MSCs possessed the characteristics of allogeneic immunological tolerance and immunoloregulation effect.

关 键 词：人脐带源间充质干细胞 同种异体 免疫耐受 免疫调节 细胞移植 

分 类 号：R394.2[医药卫生—医学遗传学]