急性淋巴细胞白血病的免疫分型及CD4^+CD25^+T调节细胞检测  

Immunophenotype of acute lymphoblastic leukemia and detection of CD4+CD25+ regulatory T cells

作　　者：巫翠萍[1] 覃西[1] 巫翠云[2] 朱洪[1] 周海燕[1] 

机构地区：[1]海南医学院附属医院,海南省海口市570102 [2]海口市人民医院,海南省海口市570208

出　　处：《中国组织工程研究与临床康复》2011年第45期8487-8490,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：海南省卫生厅科研立项课题项目(琼卫2008-55)~~

摘　　要：背景:在急性淋巴细胞白血病发病过程中,CD4+CD25+T调节细胞对机体免疫反应可能起着一定的调节作用。目的:观察急性淋巴细胞白血病患者的免疫分型及外周血CD4+CD25+T调节细胞的变化情况。方法:采用流式细胞仪对35例急性淋巴细胞白血病患者进行免疫分型,并检测外周血CD4+CD25+T调节细胞的数目,与18名健康对照作比较。结果与结论:急性B细胞淋巴细胞白血病22例,急性T细胞淋巴细胞白血病13例;22例急性B细胞淋巴细胞白血病中CD19的阳性表达率最高(100%),而13例急性T细胞淋巴细胞白血病中CD7阳性表达率最高(100%)。急性B细胞淋巴细胞白血病患者外周血CD4+CD25+T调节细胞和急性T细胞淋巴细胞白血病患者差异无显著性意义(P>0.05),但均高于健康对照(P<0.05)。表明急性B细胞淋巴细胞白血病中CD19阳性表达率最高,急性T细胞淋巴细胞白血病中CD7阳性表达率最高,同时急性淋巴细胞白血病患者外周血CD4+CD25+T调节细胞水平显著增高。BACKGROUND：CD4＋CD25＋ regulatory T cells（Treg） may have a certain regulatory effect on immune response in the pathogenic process of acute lymphoblastic leukemia（ALL）.OBJECTIVE：To explore the immunophenotype and the changes of Treg in the peripheral blood of patients with ALL.METHODS：The immunophenotype and levels of CD4＋CD25＋Treg in the peripheral blood were detected using flow cytometry in 35 ALL patients in comparison with the health control people.RESULTS AND CONCLUSION：There were 22 B-ALL cases and 13 T-ALL cases among 35 ALL patients.CD19 expression of the cases of B-ALL had the highest positive rate（100%）;meanwhile,CD7 expression of the cases of T-ALL had the highest positive rate（100%）.There were no significant difference between the number of CD4＋CD25＋ Treg cells in B-ALL and T-ALL patients（P 0.05）,but there were significant difference between the number of Treg cells in health control people and ALL patients（P 0.05）.The results show that CD19 expression of the cases of B-ALL and CD7 expression of the cases of T-ALL had the highest positive rate;the levels of CD4＋CD25＋ Treg cells significantly increased in the peripheral blood of ALL patients.

关 键 词：急性淋巴细胞白血病 流式细胞仪 免疫分型 外周血 CD4+CD25+T调节细胞 

分 类 号：R394.2[医药卫生—医学遗传学]