异基因间充质干细胞在体内免疫调节作用的时间及剂量依赖性  

Time- and dose-dependent immunomodulatory effect of allogeneic mesenchymal stem cells in vivo

作　　者：朱希山[1] 施薇[1] 台卫平[1] 张舸[1] 安广宇[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院(北京大学第九临床医学院)肿瘤内科,北京市100038

出　　处：《中国组织工程研究与临床康复》2011年第45期8499-8502,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:异基因间充质干细胞移植时,发挥作用需要的剂量、持续的时间及是否具有不良反应是目前研究的热点。目的:观察异基因的骨髓间充质干细胞在体内免疫调节作用的时间、剂量依赖性。方法:免疫系统正常的BALB/c小鼠模型,制备骨髓来源间充质干细胞,24只BALB/c小鼠随机分为4组,实验组每只小鼠分别通过尾静脉注射0.3mL细胞悬液,分别含5×105,5×104,5×103骨髓间充质干细胞,对照组小鼠仅注射0.3mL生理盐水。进行淋巴细胞增殖检测、混合淋巴细胞反应、间充质干细胞移植对异基因小鼠免疫系统的影响等实验。结果与结论:不同剂量的C57BL/6骨髓来源间充质干细胞经尾静脉注射给BALB/c受体小鼠后,骨髓间充质干细胞体内对免疫系统的调节呈剂量依赖性。间充质干细胞促进异基因移植物的植入,同时这种免疫下调作用在2周时最强,1个月逐渐减弱,2个月基本消失,提示间充质干细胞的免疫调节作用具有剂量依赖性,并且只能维持一段时间。BACKGROUND：Allogeneic mesenchymal stem cells transplantation is widely used in clinics,but the required dose,duration and whether the side effects exists are still a research hotspot.OBJECTIVE：To study the time-and dose-dependent immunomodulatory effect of allogeneic bone marrow mesenchymal stem cells in vivo.METHODS：Bone marrow-derived mesenchymal stem cells were isolated from mice with normal immune system.Twenty-four BALB/c mice were randomly divided into four groups.In experimental group,each mouse was injected with 0.3 mL cells suspension containing 5×105,5×104,5×103 bone marrow-derived mesenchymal stem cells via the tail vein,respectively.In control group,the mice were only injected with 0.3 mL normal saline.The lymphocyte proliferation test,mixed lymphocyte reaction and experiments about the effect of bone marrow-derived mesenchymal stem cells transplantation on the immune system of allogeneic mice were performed and detected.RESULTS AND CONCLUSION：After cell transplantation,bone marrow-derived mesenchymal stem cells exerted the in vivo immunomodulatory effect in a dose-dependent manner.Mesenchymal stem cells could promote the allogeneic graft implantation,and the immunomodulatory effect reached the peak at the 2nd week,gradually decreased at the 1st month and disappeared at the 2nd month.It is indicated that the immunomodulatory effects of mesenchymal stem cells are presented in a dose-dependent manner,which can only be maintained for some time.

关 键 词：间充质干细胞 异基因 免疫调节 剂量 骨髓 

分 类 号：R394.2[医药卫生—医学遗传学]