大鼠涎腺放射损伤模型的建立及NBS1的表达变化  

Expression and significance of NBS1 in irradiation injured salivary gland in rats

作　　者：林丹[1] 王代友[1] 杨亦萍[1] 卿海云[1] 曹阳[1] 陈超梅[1] 沈洁[1] 欧健波[1] 

机构地区：[1]广西医科大学口腔医学院口腔颌面外科,广西南宁530021

出　　处：《临床口腔医学杂志》2011年第12期721-724,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金项目(30960420);广西医疗卫生重点科研课题(桂卫重2010082)

摘　　要：目的:研究大鼠涎腺放射损伤模型中NBS1基因的表达,初步探讨其在放射性涎腺上皮细胞损伤修复中的调控作用。方法:放射组40只大鼠以60Coγ射线分次照射,每次3GY,隔天1次,共5次,累积剂量分别为3、6、9、12、15 GY,对照组40只大鼠同期进行麻醉。照射后2～4 h,收集大鼠腮腺,颌下腺。应用苏木素-伊红染色(HE)和透射电镜观察涎腺组织的显微和超微结构变化;应用逆转录聚合酶链技术(RT-PCR)检测腮腺、颌下腺NBS1mRNA的表达情况。结果:腮腺较颌下腺组织损伤严重;放射组和正常组比较,腮腺放射剂量9 GY组起,颌下腺于12 GY组起,NBS1mRNA表达水平减少(P<0.05)。结论:大鼠涎腺放射损伤模型建立成功,NBS1可能参与涎腺放射损伤修复。Objective： To investigate the expression of NBS1 genes in the radiation-injured model of salivary gland in rats, and preliminary explore the irradiated injury repair mechanism of NBS1 in salivary gland. Method： The rats were randomly divided into two groups, the irradiated rats （n=40） were fractionated exposed to a dose of 3Gy ^60Co γ-rays, given two days time , leading to an calculated dose of 3,6, 9, 12, 15GY; The sham-irradiated controls （n=40） were anesthetized in parallel with the irradiated rats but not irradiated. After irradiation in two to four hours,the rats were sacrificed, the histomorphological changes were observed by HE staining;the ultrastructural changes were observed by transmission electron microscopy; RT-PCR were used to detect the NBSlexpression. Result：The organization were damaged even more serious in parotid gland than in submandibular gland; Compared with the controls,with the groups of dose above 9Gy in parotid gland and the dose above 12GY in the submandibular gland, the expression of NBS1 mRNA were reduced （P 〈 0.05）.Conclusions：The model of irradiated injury salivary gland was successfully constructed, NBSI might be involved in salivary gland irradiated injury repair.

关 键 词：NBS1:MRN(MRE11-RAD50-NBS1)复合物 DNA双链断裂(DSB) 涎腺上皮细胞 放射性损伤修复 

分 类 号：Q786[生物学—分子生物学] R782.7[医药卫生—口腔医学]