联合应用MAGE-1与IL-18基因疫苗体外抗肝癌作用的实验研究  

Experiment study of anti-cancer effect of combined vaccine of MAGE-1 gene and IL-18 factor in vitro

作　　者：杨广民[1] 段秀梅[2] 李首庆[1] 马寅芙[1] 谭岩[1] 

机构地区：[1]吉林省人民医院检验科,吉林长春130021 [2]吉林大学第一医院病理科,吉林长春130021

出　　处：《中国现代医学杂志》2011年第31期3872-3875,3880,共5页China Journal of Modern Medicine

基　　金：吉林省科技发展计划项目(No:200705449);吉林省卫生厅重点实验室项目(No:200905192)

摘　　要：目的该研究构建重组基因疫苗pcDNA-MAGE-1和pcDNA-hIL-18;探讨联合应用基因疫苗体外抗肝癌免疫治疗的作用。方法基因疫苗分组免疫实验小鼠,在体外验证疫苗免疫后的小鼠脾细胞对MAGE-1阳性和阴性肝癌细胞株的杀伤作用;分别以不同剂量的pcDNA-hIL-18与pcDNA-MAGE-1组合测定免疫鼠脾细胞杀伤率,验证产生高杀伤效应的最佳的pcDNA-hIL-18浓度。结果重组基因疫苗免疫组小鼠脾细胞对两种MAGE-1阳性肿瘤细胞(SMMC-7721、BEL-7402)均有明显的杀伤作用,其杀伤效应与生理盐水组(空白对照),空质粒pcDNA3组(阴性对照)相比差异具有显著性(P<0.01);重组基因疫苗免疫后的小鼠脾细胞对MAGE-1阴性肿瘤细胞(QQY-7701)有一定的杀伤作用,与空白对照及阴性对照免疫组相比差异有显著性(P<0.05);pcDNA-hIL-18+pcDNA-MAGE-1组以(50+100)μg剂量免疫小鼠的脾细胞杀伤效应最高,与pcDNA-MAGE-1和pcDNA-hIL-18组相比差异有显著性(P<0.01)。结论联合应用MAGE-1与IL-18基因疫苗对MAGE-1阳性肿瘤细胞有明显的杀伤作用。【Objective】 To construct recombination gene vaccine pc-MAGE-1 and pc-hIL-18 and to probe anti-cancer effect of the liver of the combined genetic vaccine in vitro.【Methods】 Genetic vaccines were immunized to mice,whose spleen cells were seen as effector cell,and SMMC-7721,BEL-7402,QQY-7701 hepatoma carcinoma cells as target cell,then lethal effect of spleen cell to MAGE（＋） and MAGE（-） tumor cells were validated.Kill ratio of different dose pcDNA-hIL-18 ＋ pcDNA-MAGE-1 was detected for the best immune dose.【Results】 Genetic vaccine groups had an apparente lethal effect on MAGE（＋） tumor cells,and they had significant deviation from control groups（P 0.01）.Kill ratio obviously heightened（P 0.05）,lethal effect of pcDNA-hIL-18 ＋ pcDNA-MAGE-1 group was obviously higher than other groups,it was statistically significantly different to pcDNA-MAGE-1 group（P 0.05）.Meanwhile,genetic vaccine groups had determinate lethal effect on MAGE（-） tumor cells,and they had a statistically significant difference from control groups（P 0.05）.Kill ratio of pcDNA-hIL-18 ＋ pcDNA-MAGE-1（50＋100）μg group was highest and was statistically significantly different to pcDNA-MAGE-1 and other groups（P 0.01）.【Conclusions】 Lethal effect of pcDNA-hIL-18 ＋ pcDNA-MAGE-1 group is highest on MAGE（＋） tumor cells,and combination of IL-18 and MAGE-1 can further reinforce immuno-competence of vaccine.

关 键 词：基因疫苗 MAGE-1 IL-18 肝癌细胞 

分 类 号：R735.7[医药卫生—肿瘤]