塔里木马鹿再生茸组织中原癌基因c-myc、c-fos的表达  

Expression of Proto-oncogene c-fos,c-myc in Different Growth Stages of Reborn Antlers of Tarim Deer's Antler

作　　者：韩春梅[1,2] 高庆华[1,2] 唐继伟[1] 李杰[3] 孙献英[1] 马瑛[1] 

机构地区：[1]塔里木大学动物科学学院,新疆阿拉尔843300 [2]兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300 [3]华中农业大学动物遗传育种与繁殖教育部重点实验室,武汉430070

出　　处：《中国农学通报》2011年第29期9-12,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然基金项目"马鹿生茸期原癌基因c-myc;fos的表达调控"(30860188)

摘　　要：为进一步确定原癌基因c-myc、c-fos对马鹿茸生长的作用,实验收集231头2至4岁公鹿产茸量的生产信息,并采用3头成年马鹿生长期为30天、60天的头茬鲜茸和3头成年马鹿割后生长30天、60天的再生鲜茸,通过荧光定量PCR技术对不同组织基因的表达定量分析。结果表明,在鹿茸生长早期c-myc在再生茸茸皮的表达量显著低于头茬茸(P<0.01)。c-myc基因在割后再生的60天鹿茸茸皮和软骨组织表达下调,与头茬茸60天的相比,表达有显著差异(P<0.05)。间充质细胞层和成软骨层c-myc基因的表达量很低,且表达没有差异。从30天到60天,c-fos基因割后再生的马鹿茸茸皮中的表达下调,而在软骨组织中表达上调。与头茬60天茸相比,表达均有显著差异(P<0.05)。而在间充质细胞层和成软骨层没有差异。c-fos基因头茬与割后再生的60天马鹿茸的表达趋势与生长期为30天的相同。与头茬茸比较,再生茸成软骨层组织c-fos基因表达有上调趋势。结论:原癌基因c-myc、c-fos对茸皮的生长主要是促进其表皮干细胞的增殖分化;而对软骨组织c-myc基因高表达有阻碍茸软骨骨化,减缓骨化进程的作用,c-fos基因低表达有抑制生茸期骨膜内生骨的过程。c-fosc-myc Tarim wapitis were collected.And the first-cut antler and reborn fresh velvet antlers of 3 adult Tarim wapitis with 30 or 60-day-old were used to be experimental material.Quantitative expression in different zones of antler were determined by fluorescent quantitative PCR.The results demonstrated that the expression of c-myc in reborn dermal layer of antler early growth was lower than that in the first-cut antlers （P0.01）.The gene c-myc was down regulated in dermal layer and cartilage layer of the reborn antler with 60-day-old,and compared to 60 days of natural growth antler,it was significant difference in both of them （P0.05（The low expression of c-myc was observed in mesenchymal cells layer and precartilage cells layer in different growth period.Dropped expression of c-fos was observed in dermal layer of reborn antler while upward expression in cartilage layer from 30 days to 60 days and there was significant difference compared to natural growth antler （P 0.05）.Little variation was found between the expressions of c-fos in mesenchymal cells layer and precartilage cells layer.Express of c-fos in reborn antlers with 60-day-old was similar to that the first-cut antler with 30 days,and c-fos was down regulated in cartilage layer of the reborn antler.The results suggested that c-myc and c-fos mainly promote the proliferation and differentiation of stem cells in dermal layer during the rapid growth of the antlers while in cartilage layer high expression of c-myc might inhibit cartilage osteoblast and low expression of c-fos inhibited process of Periosteal endogenous bone in antler ＇ s growth period.

关 键 词：塔里木马鹿 再生茸 原癌基因 C-MYC C-FOS 

分 类 号：S813.2[农业科学—畜牧学]