胃癌组织中Fas/FasL表达及T淋巴细胞亚群分析  

The Study about the Expression of Fas/FasL and T Lymphocyte Subsets in Gastric Carcinoma Tissue

作　　者：欧建锋[1] 任晖[2] 李莎[3] 

机构地区：[1]兰州军区兰州总医院血液科,兰州730050 [2]兰州军区兰州总医院乳腺科,兰州730050 [3]兰州军区兰州总医院放疗科,兰州730050

出　　处：《临床误诊误治》2011年第12期13-15,共3页Clinical Misdiagnosis & Mistherapy

基　　金：甘肃省科技攻关项目(2GS064-A43-020-23)

摘　　要：目的探讨Fas/FasL和T淋巴细胞亚群在肿瘤免疫逃逸中的作用。方法采用流式细胞术对经病理检查确诊为胃癌的癌组织、癌旁组织、转移性淋巴结进行Fas/FasL表达测定和T淋巴细胞亚群分析。结果 CD4+细胞在癌组织中的表达低于癌旁组织及转移性淋巴结,差异有统计学意义(P<0.01),癌旁组织与转移性淋巴结两者间差异无统计学意义(P>0.05);CD8+细胞在癌组织中的表达均高于癌旁组织及转移性淋巴结,差异有统计学意义(P<0.01),癌旁组织与转移性淋巴结两者间差异无统计学意义(P>0.05)。Fas在癌旁组织及转移性淋巴结中呈高表达,两者间差异无统计学意义(P>0.05),但在癌组织中呈低表达,癌组织与癌旁组织及转移性淋巴结之间比较差异有统计学意义(P<0.01)。FasL在癌组织及转移性淋巴结均呈高表达,两者间差异无统计学意义(P>0.05),与癌旁组织比较差异有统计学意义(P<0.01)。结论胃癌患者FasL诱导激活的T淋巴细胞凋亡,使肿瘤细胞可能逃逸机体免疫系统攻击。Objective To explore the effect of Fas/FasL and T lymphocyte subsets in immune escape of neoplasm. Methods Flow cytometry was used to detect T lymphocyte subsets and Fas/FasL in tumorous tissue or peri-tumorous tissue and metastasis tissue of lymph node were derived from gastric cancer patients. Results The expression of CD4＋ cells in tumorous tissue was lower than that in peri-tumorous tissue and metastasis tissue of lymph node （P 〈 0. 01）, without significant difference between peri-tumorous tissue and metastasis tissue of lymph node （P〉0. 05）. The expression of CD8＋ ceils was higher in tumorous tissue than that in peri-tumorous tissue and metastasis tissue of lymph node（P 〈0. 01）, without significant difference between peri-tumorous tissue and metastasis tissue of lymph node （P 〉 0. 05）. The expression of Fas ＋ cells was higher in peri-tumorous tissue and metastasis tissue of lymph node than that in tumorous tissues（P 〈0. 01 ）, but without significant difference between peri-tumorous tissue and metastasis tissue of lymph node （P 〉 0. 05）. The expression of FasL ＋ cells was higher in tumorous tissue and metastasis tissue of lymph node than that in peri-tumorous tissue （P 〈 0. 01 ）, but without significant difference between tumorous tissue and metastasis tissue of lymph node （P 〉 0. 05）. Conclusion FasL may induce apoptosis of activated T lymphocyte, so that tumor cells may escape from being attacked by immune system in gastric carcinoma.

关 键 词：胃肿瘤 肿瘤逃逸 FAS FASL T淋巴细胞亚群 

分 类 号：R735.2[医药卫生—肿瘤]