结合缺陷型菌株显色法采用离子束诱变筛选高产L-精氨酸突变株  

Mutated by iron implantation for screening high L-arginine production strains using a plate coloration method by auxotroph strain

作　　者：杨娟[1] 饶志明[1] 徐美娟[1] 夏海锋[1] 窦文芳 许正宏 

机构地区：[1]江南大学工业生物技术教育部重点实验室应用微生物与代谢工程研究室,江苏无锡214122 [2]江南大学医药学院,江苏无锡214122

出　　处：《微生物学通报》2011年第12期1820-1827,共8页Microbiology China

基　　金：教育部新世纪优秀人才计划项目(No.NCET-10-0459);国家973计划项目(No.2007CB707804);国家863计划项目(No.2007AA02Z207);国家自然科学基金项目(No.30970056);中央高校基本科研业务费专项资金项目(No.JUSRP31001);教育部111引智计划项目(No.111-2-06);江苏省优势学科工程项目

摘　　要：为快速高效筛选L-精氨酸高产突变株,建立一种缺陷菌株平板显色法并采用低能N+离子束对L-精氨酸生产用菌株钝齿棒杆菌SYPA5-5进行诱变处理,通过上述平板显色法筛选获得高产突变株。对突变株进行摇瓶发酵实验,最终选育出一株L-精氨酸产量较高且产酸性能比较稳定的突变菌株钝齿棒杆菌SYPA5-5-36。该菌株摇瓶发酵L-精氨酸产量可达35.85 g/L,比出发菌株提高了19.5%。因此,缺陷型菌株平板显色法可以用于快速、高效筛选高产L-精氨酸突变株。A plate coloration method by auxotroph strain for screening high L-arginine production mutants quickly and efficiently was established. Coynebacterum crenatum SYPA5-5 which is an L-arginine producer was mutated by N＋ ion implantation with an energy level of 10 keV. Combined with the directed screening method and fermentation in flasks, a mutant C. crenatum SYPA5-5-36 with a higher L-arginine production and stability was selected. The yield of L-arginine produced by this mutant could reach 35.85 g/L which was 19.5% higher than that of the original strain. Therefore, the plate coloration method by auxotroph strain could be applied to screening high L-arginine production mutants quickly and efficiently.

关 键 词：L-精氨酸 离子束注入 钝齿棒杆菌 营养缺陷型 筛选 

分 类 号：TQ922.9[轻工技术与工程—发酵工程]