茶树DNA的快速抽提法  

A Simple and Rapid DNA Extraction Method of Tea

作　　者：陈志辉[1] 钟秋生[1] 林郑和[1] 游小妹[1] 陈常颂[1] 

机构地区：[1]福建省农业科学院茶叶研究所,福建福安355000

出　　处：《湖北农业科学》2011年第23期4962-4964,共3页Hubei Agricultural Sciences

基　　金：现代农业产业技术体系建设专项资金资助(CARS-23);福建省属公益类科研院所基本科研专项(2010R1014-4);福建省农科院创新团队资助项目

摘　　要：使用简化改良的CTAB法抽提茶树(Camellia sinensis)鲜叶DNA,通过电泳和PCR检测所提取DNA的质量。实验结果表明,改良的CTAB法提取茶树DNA,步骤减少、抽提过程简单、时间短、效率高,用嫩叶和成熟叶均能抽提出高质量的DNA。CTAB method was simplified and meliorated to extract genome DNA from tea leaves,and the product was examined by electrophoresis and PCR.Results showed that preservation time of branches in water had no obvious effect on the quality of DNA extracted.The modified CTAB method was simple and fast as DNA with high quality could be obtained not only from tender leaves but also from mature and old leaves.

关 键 词：茶树(Camellia sinensis) DNA快速抽提 CTAB法 鲜叶保存 

分 类 号：S571.1[农业科学—茶叶生产加工] Q523[农业科学—作物学]