口腔粘膜白斑和鳞癌组织中p53和bcl-2蛋白的表达  

Expression of bcl-2、p53 protein in oral leukoplakia and squamous cell carcinoma

作　　者：关为群[1] 于世凤[1] 高岩[1] 

机构地区：[1]北京医科大学口腔医学院口腔病理研究室,100081

出　　处：《临床与实验病理学杂志》1999年第6期480-483,I068,共5页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的：研究凋亡相关蛋白bcl-2、p53在口腔正常粘膜、上度异常增生和鳞癌组织中的表达及意义。方法：采用免疫组织化学S－P法检测10例正常口腔粘膜、10例单纯性增生上皮、30例异常增生上皮和33例鳞癌石蜡包埋组织中bcl－2、p53的表达。结果：正常粘膜和上皮单纯增生组织中未见p53阳性表达。异常增生上皮和鳞癌组织中p53阳性率分别为33．33％和45．45％，与正常组和单纯增生组相比有显著性差异（P＜0．05）。bcl-2在正常粘膜、单纯增生和异常增生白斑表达情况基本一致，显弱阳性表达且局限于基底层细胞，鳞癌组织中bcl－2表达明显高于正常组和白斑组（P＜0．05）。两种指标的进一步相关分析显示，癌前病变组两种蛋白的出现无相关，鳞癌组p53和bcl－2的表达呈明显的正相关。结论：口腔癌发生的早期p53基因作用较明显，bcl－2基因则在口腔癌发生的后期和突变型P53共同作用，在促进肿瘤细胞异常增殖的同时，进一步抑制细胞的凋亡导致肿瘤的发生。Purpose To study the expression and signficance of apoptosis-related protein bcl-2, p53 in oralnormal mucosa, leukoplakia (LK) and squamous cell carcinoma (SCC). Methods The expression was observedin 10 normal oral epithelial, 10 KL with simple hyperplasia, 30 LK with epithelial dysplasia and 33 SCC by im-munohistochemical evaluation. Results No p53 expression was located in normal mucosa and simple hyperplasiaLK, but there had a higher percentage of its immunoreactivity in dysplasia LK and SCC (P < 0. 05 ); In normalmucosa and LK, the positive bcl-2 expression was similar (P >0. 05 ), and they almost confined to basialis cell.There had a significant elevation of bcl-2 exepression in SCC(P < 0. 05 ). Further analysis showed the correlationwas evident between expression of these two proteins in SCC but not in LK. Conclusion The occurence of oraldysplastic LK mainly resulted from p53 gene alteration, the cooperation of abnormal p53 and bcl-2 expression re-sulted in the progression of SCC.

关 键 词：口腔粘膜白斑 口腔鳞癌 P53蛋白 BCL-2蛋白 

分 类 号：R739.850.2[医药卫生—肿瘤]