噻虫胺胁迫对小白菜中谷胱甘肽和蛋白质含量的影响  

Effect of clothianidin stress on the content of Protein and GSH in Brassica pekinensis

作　　者：陈雁君[1] 王艳 程晓平[1] 郭建丽[1] 张建萍[1] 赵艳霞[1] 

机构地区：[1]济宁医学院医学检验系,山东济宁272067 [2]临沂市蒙阴县坦埠医院,山东临沂276200

出　　处：《济宁医学院学报》2011年第6期395-397,共3页Journal of Jining Medical University

摘　　要：目的寻找较敏感的生物标记物,作为噻虫胺污染蔬菜的早期诊断指标。方法谷胱甘肽(GSH)含量测定采用Ellman法。可溶性蛋白质含量测定采用考马斯亮蓝。结果在胁迫初期GSH含量急剧降低,低浓度处理组GSH含量在第5天下降到最低点,高浓度处理组GSH含量在第7天下降到最低点。处理组的蛋白质含量均明显低于对照组,浓度越高蛋白质含量越低,与对照组有显著性差异。结论 GSH和蛋白质均可作为噻虫胺污染蔬菜的早期敏感生物检测指标。Objective To study the effect of Clothianidin stress on the content of GSH and Protein in Brassica pekinensis. Methods The content of GSH and Protein in Brassica pekinensis is determined by Coomassie brilliant blue and Ellman methods. Results The results showed that the synthesis of GSH and protein was suppressed under the Clothianidin stress in the high and low concentration groups. The concentrations in study groups were lower than controls significantly. At last,it is consistent with the control group. Conclusion The content of GSH and protein in Brassica pekinensis suffered Clothianidin stress changes significantly in early time. So it could be served as sensitive biomarker under Clothianidin stress in Brassica pekinensis.

关 键 词：噻虫胺 GSH 蛋白质 小白菜 

分 类 号：R155.54[医药卫生—营养与食品卫生学]