响应面法优化红曲霉胞外多糖提取工艺  

Optimizing the Extraction Technology of Monascus Exopolysaccharide by Response Surface Methodology

作　　者：汪鹏荣[1] 蔡琪敏[1] 崔宇辉[1] 蒋冬花[1] 陈丹凤[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,金华321004

出　　处：《中国粮油学报》2011年第12期83-87,92,共6页Journal of the Chinese Cereals and Oils Association

基　　金：国家自然科学基金(31070008)

摘　　要：对实验室自行分离得到的紫色红曲霉MP-66发酵液中的胞外多糖提取条件进行优化,以提高胞外多糖的产量。通过单因素试验,研究了乙醇体积分数、乙醇沉淀时间、发酵滤液pH对红曲霉胞外多糖的得率的影响,并在单因素试验基础上,设计三因素三水平的Box-Behnken中心组合试验及响应面法分析对红曲霉胞外多糖的提取工艺进行优化,建立了二次多项式回归方程的预测模型,结果表明:乙醇沉淀时间为16.5 h,乙醇体积分数为80%,发酵滤液pH为8.05,在此条件下红曲霉胞外多糖得率可达7.40 g/L。The extraction conditions for producing exopolysaccharide by Monascus purpureus MP - 66 were optimized in order to enhance the extraction yield. Single - factor experiments were carried to investigate the influences of the alcohol volume fraction, the sedimentation time of alcohol, and the pH value of fermentation broth on extraction yield of Monascus exopolysaccharide. On the basis of single - factor experiments, a quadratic polynomial regression equation forecasting model was set up by designing a Box - Behnken central composite experiment involving of three factors and three levels and the method of response surface analysis, thus the optimized extraction conditions for Monascus exopolysaccharide were obtained. According to the results, alcohol sedimentation time was 16.5 h, the alco- hol column fraction was 80%, and the pH value of fermentation broth was 8.05. Based on these conditions, the yield of Monascus exopolysaccharide could reach 7.40 g/L.

关 键 词：胞外多糖 提取工艺 单因素试验 响应面分析法 

分 类 号：Q939.9[生物学—微生物学]