西方蜜蜂一个新表皮蛋白基因的克隆和结构分析  

Cloning and Characterization of a Novel Cuticular Protein Gene from Apis Mellifera

作　　者：欧阳昊[1] 葛清秀[2] 孙亮先[2] 梁勤[1] 

机构地区：[1]福建农林大学蜜蜂分子生物学实验室,福建福州350002 [2]泉州师范学院分子生物学与药物化学重点实验室,福建泉州362000

出　　处：《泉州师范学院学报》2011年第6期1-6,共6页Journal of Quanzhou Normal University

基　　金：国家自然科学基金项目(31072095);福建省教育厅省属高校项目(JA09214)

摘　　要：利用西方蜜蜂雄蜂头部5’LongSAGE文库中的一组标签序列,在蜜蜂基因组序列第9号连锁群上定位一个新的西方蜜蜂表皮蛋白基因转录起始位点,并克隆了该基因的cDNA序列,继而利用此cDNA序列分析了该基因的内含子和外显子结构.分析结果显示:该基因的DNA序列长1 253bp,含有4个外显子和3个内含子,其cDNA长598bp,编码一个169AA的富含Val和Pro残基(23.67%,20.12%)多肽序列.BLAST分析发现,该蛋白与已知的4个昆虫表皮蛋白序列的相似性为47.54%,且其C末端有一个共有基序,说明这5个蛋白可能属于同一个表皮蛋白家族.该研究结果提供了一个新的蜜蜂表皮蛋白基因,且该基因在雄蜂头部表达水平较高.In this study,we mapped the transcription start site （TSS） of a novel cuticular protein gene from Apis mellifera with a group of 5＇ LongSAGE tag sequences, and cloned its full-length eDNA sequence. The genomic DNA sequence of this novel gene is 1 253 bp,containing four exons and three introns. Its full-length eDNA is 598 bp, encoding a 169 AA polypeptide sequence abundant of Val and Pro residues（23. 67%, 20. 12%）. Multialignment analysis reveals that the similarity of deduced amino acid sequence with four reported insect cutieular proteins is 47. 54%, and shares a common motif at C-terminal of the cuticular protein , indicating they are likely to be a member of a euticular protein family. Our 5＇ LongSAGE data show this gene is highly expressed in drone head of this study. This results of this study provided a novel cutieular protein gene of honeybee.

关 键 词：西方蜜蜂 5’LongSAGE 表皮蛋白 转录起始位点 基序 

分 类 号：Q966[生物学—昆虫学]