肿瘤坏死因子基因6个SNPs与河南汉族原发性高血压的相关性研究  

Correlation between TNF α and β Gene Polymorphisms and Essential Hypertension in HAN Chinese of Henan Province

作　　者：郭利伟[1] 李端[2] 黄艳梅[1] 冯志博[1] 康利霞[3] 

机构地区：[1]新乡医学院基础医学院,河南453003 [2]新乡医学院生命科学技术系 [3]新乡医学院第三附属医院检验科

出　　处：《中国慢性病预防与控制》2011年第6期571-573,共3页Chinese Journal of Prevention and Control of Chronic Diseases

基　　金：河南省教育厅自然科学基金(2008A310010)

摘　　要：目的探讨肿瘤坏死因子基因6个SNPs位点多态性与河南汉族原发性高血压(EH)的相关性。方法采用限制性扩增片段长度多态性(PCR-RFLP)的方法检测TNF-α启动区基因-238G>A、-308G>A、-850C>T、-857C>T、-863C>A及TNF-β第一内含子基因+252G>A多态性,并运用卡方检验探讨各SNP位点多态性与EH的相关性。结果 TNF-α-308G>A位点基因型分布和等位基因频率在人群中存在差异显著性(χ2=6.407,P=0.041;χ2=4.781,P=0.029),EH组中,TNF-α-308G>A中基因型GA+AA人群的收缩压(SBP)、舒张压(DBP)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、尿酸(UA)及空腹血糖(FPG)含量明显高于GG基因型人群(P<0.05);TNF-α基因-238G>A、-850C>T、-857C>T、-863C>A及TNF-β基因+252G>A多态性各基因型及等位基因频率在EH组和对照组的分布差异无统计学意义。结论 TNF-α基因-308G>A位点基因多态性与河南汉族原发性高血压的发病相关。TNF-α-238G>A、-850C>T、-857C>T、-863C>A及TNF-β+252G>A位点基因多态性与河南汉族原发性高血压无关联。Objective To investigate the correlation between 6 SNPs on tumor necrosis factor gene and essential hypertension （EH） in Han Chinese in Henan province. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used to evaluate the gene polymorphisms including TNF-α-238G〉A, -308G〉A, -850C〉T, -857C 〉T, -863C 〉A gene and TNF-β ＋252G〉A gene in 260 unrelated EH patients （EH group） and 394 healthy population （control group）. Results The frequencies of the GA ＋AA genotype and allele of TNF-α-308G 〉A in EH patients were significantly higher than those in heahh control group （x2=6.407andx2=4.781,allP〈0.05）,inEHpatients, theSBP, DBP, HDL, LDL, UA and FGB values were significant difference between the GA＋AA genotype and GG genotype subgroups （P〈0.05）. There was no significant difference in genotype and allelic frequency of the TNF-α-238G〉A, -850C〉T, -857C〉T, -863C〉A gene and TNF-β ＋252G 〉A polymorphism between healthy subjects and EH patients. Conclusion The TNF-α-308G 〉A gene polymorphism is positively eorrelated with EH of Han Chinese in Henan. And the TNF-α-238G〉A, -850C〉T, -857C〉T, -863C〉A gene and TNF-β＋252G〉A gene polymorphism is not associated with the susceptibility to EH.

关 键 词：肿瘤坏死因子 原发性高血压 单核苷酸多态性 河南汉族 

分 类 号：R544.1[医药卫生—心血管疾病]