雷帕霉素诱导自噬抑制舌鳞癌细胞增殖和迁移  

Rapamycin induced autophagy and suppression in tongue squamous cell carcinoma cell proliferation and migration

作　　者：王雅雯[1] 唐海阔[1] 汪淼[1] 王成[1] 翁军权[1] 侯劲松[1] 

机构地区：[1]中山大学光华口腔医学院附属口腔医院口腔医学研究所,广州510055

出　　处：《中华口腔医学研究杂志（电子版）》2011年第6期4-6,共3页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：广东省自然科学基金(10151008901000025);广东省科技计划(2009B050700024;2009B060700096)

摘　　要：目的探讨自噬诱导对舌鳞癌细胞增殖和迁移能力的影响。方法应用雷帕霉素(Rap)诱导上调舌鳞癌Tca8113细胞自噬活性,Western blot检测诱导后Tca8113细胞自噬标记蛋白Beclin1和LC3的表达变化;MTT法检测自噬诱导对细胞增殖的影响;Wound-healing检测诱导后细胞迁移能力改变;SPSS 19.0统计软件进行数据分析。结果 Rap诱导6h即可上调舌鳞癌Tca8113细胞自噬标记蛋白LC3-Ⅱ表达,24h时LC3-Ⅱ表达最强,自噬活性最高(P<0.05)。与对照组相比,Rap诱导后细胞生长明显抑制(P<0.05),迁移速率减慢。结论上调舌鳞癌细胞自噬活性可以抑制其增殖和迁移。Objective To investigate the effect of autophagy induced by rapamycin on proliferation, migration of tongue squamous cell carcinoma （TSCC） cell line. Methods TSCC cell line （Tca8113） was treated with rapamycin to induce autophagy. Then, Western blot was performed to examine the expression of Beclinl and LC3. MTF assay was used to evaluate cell proliferation. Wound healing was carried out to assess cell migration. Results After 6 h of Rapamycin treatment, the expression of LC3 was gradually increased in Tca8113 cell. After treatment with rapamycin for 24 h, LC3-H was dramatically increased at protein level and autophagical activity was significantly increased （P 〈 0.05）. MTT assay indicated that cell proliferation was inhibited by Rapamycin. Migration ability was also reduced after treatment with rapamycin （P 〈 0.05）. Conclusion Rapamycin can induce autophagy and suppress proliferation and migration in tongue squamous cell line.

关 键 词：雷帕霉素 自噬 舌鳞癌细胞 增殖 迁移 

分 类 号：R739.86[医药卫生—肿瘤]