口腔鳞状细胞癌Tca8113细胞中透明质酸酶的表达及意义  

The significance of HAase expression in Tca8113 cell

作　　者：王敬旭[1] 刘海潮[2] 廖贵清[2] 

机构地区：[1]广州医学院第一附属医院口腔科,510120 [2]中山大学光华口腔医学院附属光华口腔医院口腔医学研究所

出　　处：《中华口腔医学研究杂志（电子版）》2011年第6期7-9,共3页Chinese Journal of Stomatological Research(Electronic Edition)

摘　　要：目的探讨口腔鳞状细胞癌(OSCC)Tca8113细胞中透明质酸酶(HAase)的表达及意义。方法以正常人牙龈上皮细胞作对照,采用RT-PCR、免疫荧光技术检测人OSCCTca8113细胞株中HAase的mRNA水平及其蛋白表达。结果 HAase主要在Tca8113细胞和正常上皮细胞胞浆内表达;HAasemRNA的半定量检测表明,Tca8113细胞内HAase的表达强度比相对应的正常牙龈上皮细胞内HAase的表达强度显著增高,差异有统计学意义。结论细胞胞浆中HAase的表达水平可能与细胞的恶变有关。Objective To evaluate the significance of HAase expression in Tca8113 cell. Methods The expression of HAase mRNAs and proteins of Tca8113 cell and normal human gingival epithelial cell were analysised by reverse transcription polymerase chain reaction （RT-PCR）, and Immunohistochemical staining, respectivly. Results HAase were mainly stained in cell plasms. The expression of HAase mRNA in Tca8113 cell was significantly higher than that of normal human gingival epithelial cell （P 〈 0.05）. Conclusion The expression of HAase in cells is related to malignance.

关 键 词：口腔鳞状细胞癌:Tca8113细胞:透明质酸酶 

分 类 号：R739.8[医药卫生—肿瘤]