Prenatal express-diagnosis by the method of QF-PCR and automatic microelectroforesis with microarrays  

作　　者：Zaporozhan VN Bubnov VV Marichereda VG Verbitskaya TG Belous OB 

机构地区：[1]Odessa National Medical University

出　　处：《中国现代医学杂志》2011年第27期3346-3348,共3页China Journal of Modern Medicine

摘　　要：The modern molecular-genetic methods have been implementing actively into the medical practice.They improve diagnostic accuracy,help to prognosticate the course of oncological diseases,optimize the results of prenatal diagnosis,decrease mothers' anxiety and improve the clinical outcomes of pregnancy.There are used the various traditional approaches e.g.cariotyping,FISH and more contemporary-real-time PCR,comparative genomic hybridization(CGH) or chromosomal microarray analysis(CMA),Quantitative Fluorescent PCR(QF-PCR).For expressing diagnosis of triploidy by 21st and 18th chromosomes there was used QF-PCR technologies with the consequent quantative analysis on the automatic capillary microelectrophoresis on the microarrays Expеrion DNA1K.There was determined that diagnostic accuracy of QF-PCR was comparable with existing routine methods,but it had some advantages including expressity and could be recommended for implementation into practical medicine.The modem molecular-genetic methods have been implementing actively into the medical practice. They improve diagnostic accuracy, help to prognosticate the course of oncological diseases, optimize the results of prenatal diagnosis, deerease mothers＇ anxiety and improve the clinical outcomes of pregnancy. There are used the various traditional approaches e.g. cariotyping, FISH and more contemporary-real-time PCR, comparative genomic hybridization （CGH） or chromosomal microarray analysis （CMA）, Quantitative Fluorescent PCR （QF-PCR）. For expressing diagnosis of triploidy by 21st and 18th chromosomes there was used QF- PCR technologies with the consequent quantative analysis on the automatic capillary microelectrophoresis on the microarrays Experion DNA1K. There was determined that diagnostic accuracy of QF-PCR was comparable with existing routine methods, but it had some advantages including expressity and could be recommended for implementation into practical medicine.

关 键 词：医生 诊断 临床 QF-PCR 

分 类 号：R192.3[医药卫生—卫生事业管理]