槟榔碱及口腔角质形成细胞对成纤维细胞胶原酶活性的影响  

The affect on collagenase bioactivity of fibroblasts by Arecoline and oral keratinocytes

作　　者：李霞 凌天牖[2] 高义军[2] 

机构地区：[1]佛山科技学院医学院(佛山市口腔医院) [2]中南大学湘雅二医院口腔科,湖南长沙410011

出　　处：《中国现代医学杂志》2011年第27期3361-3363,3367,共4页China Journal of Modern Medicine

摘　　要：目的了解口腔粘膜下纤维性变中胶原堆积的机制。方法对口腔粘膜角质形成细胞和成纤维细胞进行了共培养,并采用槟榔碱预处理角质形成细胞,用明胶酶谱方法检测其胶原酶的含量及活性。用碱水解法检测胶原含量。结果只有共同培养组才能检测到MMP-9含量;槟榔碱预处理共同培养组的活化型MMP-2水平明显升高(P<0.05)。单独培养组中无MMP-9表达。共同培养组中胶原水平明显低于单独培养组(P<0.05)。结论 OSF组织中胶原堆积可能与MMP-2,MMP-9的表达没有明显关系。[ Objective ] To explore the mechanism of collagen cumulating in oral submucous fibrosis. [Methods ] Oral keratinocytes were pretreated by arecoline and cocultured with fibroblasts. The level of collagen, the content and activity of matrix metalloproteinase were detected.[Results] MMP-9 was produced only by co-cultm＇e group, The active form of MMP-2 in coculture groups were significantly higher than those of the non-cocuhure groups. The collagen concentration in cocuhure groups was significantly lower than that of non-coculture groups. [ Conclusion ] Collagen increasing in oral submucous fibrosis may not due to the expression of MMP.

关 键 词：槟榔碱 角质形成细胞 基质金属蛋白酶 口腔粘膜下纤维性变 

分 类 号：R781.5[医药卫生—口腔医学]