响应面法优化刺槐花黄酮类化合物的微波提取工艺  

Optimization of Microwave Extraction of Flavonoids from Robinia pseudoacacia L. Flowers Using Response Surface Methodology

作　　者：向昌国[1] 向宁[2] 黄成龙[3] 孙海滨[3] 李文芳[3] 

机构地区：[1]吉首大学生态旅游应用技术湖南省重点实验室,湖南张家界427000 [2]湖南农业大学食品科技学院,湖南长沙410128 [3]吉首大学城乡资源与规划学院,湖南张家界427000

出　　处：《食品科学》2011年第22期32-36,共5页Food Science

基　　金：生态旅游应用技术湖南省重点实验室资助项目(JDSTLY201106)

摘　　要：目的:利用响应面法对刺槐花黄酮类化合物的提取工艺进行优化。方法:在单因素试验的基础上,选择液料比、乙醇体积分数、微波时间为自变量,黄酮类化合物提取量为响应值,利用响应面分析法,研究各自变量交互作用及其对黄酮类化合物提取量的影响,模拟得到二次多项式回归方程的预测模型。结果:刺槐花黄酮类化合物的提取工艺为乙醇体积分数75%、液料比37:1(mL/g)、微波时间145s、提取两次。结论:在最佳工艺条件下黄酮类化合物提取量为8.63mg/g。The optimum conditions for the extraction of flavonoids from Robinia pseudoacacia L. flowers were determined using response surface methodology. On the basis of one-factor-at-a-time experiments, Box-Benhnken central composite design was applied to investigate the effects of three variables, namely alcohol concentration, liquid-to-solid ratio and microwave heating time on extraction yield of flavonoids. A regression model describing extraction yield of flavonoids as a function of these three variables was established. The best results of the extraction of flavonoids from Robinia pseudoacacia L. flowers were obtained when the samples were extracted twice by means of microwave heating for 145 s at a ratio of liquid to solid of 37：1 using 75% ethanol. Under these conditions, the extraction yield of flavonoids was 8.63 mg/g.

关 键 词：刺槐花 黄酮类化合物 响应面法 微波提取 

分 类 号：TQ243[化学工程—有机化工]