亚硒酸钠对子宫内膜癌细胞增殖能力的影响  

Effect of sodium selenite on proliferation of endometrial cancer cells

作　　者：郑军生[1] 周洁[1] 李小毛[1] 林琛莅[2] 

机构地区：[1]中山大学附属第三医院妇产科,广东广州510630 [2]暨南大学医学院病理学系,广东广州510632

出　　处：《中国病理生理杂志》2011年第12期2258-2264,共7页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30772332)

摘　　要：目的:研究亚硒酸钠(Na2SeO3)对子宫内膜癌细胞增殖能力的影响,并探讨其作用机制。方法:用Na2SeO3作用于子宫内膜癌Ishikawa细胞和HEC-1A细胞,MTT法检测细胞增殖能力,流式细胞法测定Na2SeO3作用后细胞周期的变化及凋亡情况,Western blotting检测周期蛋白cyclin A的表达。结果:Na2SeO3对子宫内膜癌Ishikawa细胞和HEC-1A细胞的增殖均有抑制作用,抑制率在一定浓度范围内与Na2SeO3浓度呈正相关,对Ishikawa细胞作用48 h的IC50为3.26μmol/L,对HEC-1A细胞作用48 h的IC50为4.77μmol/L。Na2SeO3作用后两种细胞G0/G1期减少,S期细胞增多,G2/M期细胞有所增加。作用48 h后两种细胞凋亡率增加。Na2SeO3使子宫内膜癌Ishikawa细胞和HEC-1A细胞的cyclin A表达增加。结论:亚硒酸钠对子宫内膜癌Ishika-wa细胞和HEC-1A细胞增殖有抑制作用,其作用机制与上调cyclin A表达,引起细胞周期阻滞和诱导凋亡有关。AIM： To investigate the effect and mechanism of sodium selenite（Na2SeO3） on the proliferation of endometrial cancer cells.METHODS： Endometrial cancer Ishikawa cells and HEC-1A cells were treated with Na2SeO3.The effect of Na2SeO3 on cell proliferation was determined by MTT assay.The effects of Na2SeO3 on cell cycle distribution and apoptosis were tested by flow cytometric analysis.The expression of cyclin A was detected by Western blotting.RESULTS： Na2SeO3 inhibited the proliferation of Ishikawa cells and HEC-1A cells.For Ishikawa cells,IC50 was 3.26 μmol/L,and for HEC-1A cells,IC50 was 4.77 μmol/L.After treated with Na2SeO3,the cells in G0/G1 phase were reduced and the cells in S phase and G2/M phase were increased.Na2SeO3 also increased the percentage of apoptosis cells.The result of Western blotting showed that the expression of cyclin A was increased.CONCLUSION： Na2SeO3 inhibits the proliferation of endometrial cancer Ishikawa cells and HEC-1A cells via up-regulating the expression of cyclin A,arresting cell cycle and inducing apoptosis.

关 键 词：亚硒酸钠 子宫内膜癌 细胞周期 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]