结缔组织生长因子在高糖诱导的足细胞上皮间充质转化中的作用  

Role of connective tissue growth factor in high glucose-induced epithelial-mesenchymal transition in podocytes

作　　者：戴厚永[1,2] 汤日宁[1] 郑敏[1] 倪杰[1] 马坤岭[1] 刘必成[1] 

机构地区：[1]东南大学附属中大医院肾内科,江苏南京210009 [2]南通大学附属医院肾内科,江苏南通226001

出　　处：《中国病理生理杂志》2011年第12期2291-2295,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30870953)

摘　　要：目的:研究结缔组织生长因子(CTGF)在高糖诱导的足细胞上皮间充质转化(EMT)中的作用。方法:将在分化条件下的足细胞分为正常葡萄糖(5 mmol/L)培养组、正常葡萄糖+CTGF(50μg/L)组、高糖(30mmol/L)培养组和高糖+CTGF组,在37℃条件下刺激24 h。用相差显微镜观察足细胞形态,分别用real-time RT-PCR和Western blotting方法检测足细胞相关EMT指标。同时用抗CTGF抗体和高糖共同培养足细胞,检测其对足细胞EMT的影响。结果:高糖培养时,足细胞表型发生改变,由正常情况下的树枝状变为鹅卵石状,CTGF与高糖共同培养,进一步使足细胞发生梭形改变。高糖作用下,足细胞发生EMT,上皮细胞标志物nephrin表达减少,间充质细胞标志物desmin表达增加,CTGF和高糖协同作用,使足细胞EMT更加明显,而抗CTGF抗体能抑制高糖诱导的足细胞EMT。结论:CTGF参与了高糖诱导的足细胞EMT的发生过程,阻断CTGF能减轻此病理变化而发挥足细胞保护作用。AIM： To investigate the role of connective tissue growth factor（CTGF） in high glucose-induced epithelial-mesenchymal transition（EMT） in podocytes.METHODS： The differentiated podocytes cultured under different conditions for 24 h at 37 ℃ were divided into 4 groups： control（5 mmol/L glucose solution）,5 mmol/L glucose solution supplemented with 50 μg/L CTGF,high glucose（30 mmol/L glucose solution） and high glucose supplemented with CTGF（50 μg/L）.The morphology of cultured podocytes was observed under phase-contrast microscope.To study the relevant markers of EMT,the mRNA and protein expression was analyzed by real-time RT-PCR and Western blotting,respectively.In addition,the effect of CTGF inhibition with anti-CTGF antibody on high glucose-induced EMT in podocytes was investigated.RESULTS： High glucose induced phenotypic transition in the podocytes from an arborization morphology to a cobblestone morphology.After addition of CTGF to high glucose culture,the podocytes developed a feature of spindle.Under high glucose condition,podocytes underwent EMT,as the epithelial marker（nephrin） was decreased and the mesenchymal marker（desmin） was increased.Exogenous CTGF showed the effect of synergy on high glucose-induced EMT.Moreover,high glucose-induced EMT in podocytes was prevented by CTGF inhibition with anti-CTGF antibody.CONCLUSION： CTGF is involved in high glucose-induced EMT in podocytes.Inhibition of CTGF might prevent podocytes from injury in diabetes.

关 键 词：结缔组织生长因子 高糖 足细胞 上皮间充质转化 

分 类 号：R363[医药卫生—病理学]