ⅡA分泌型磷脂酶A2在胃癌中的表达及其与微血管形成的关系  

Expression of Group-llA Secretory Phospholipase A2 in Gastric Carcinoma and Relationship to Microvessel Formation

作　　者：陈晖[1] 王承党[1] 庄则豪[1] 吴婷[1] 陆岽[1] 李文清[1] 陈玉丽[1] 

机构地区：[1]福建医科大学附属第一医院,福州市350004

出　　处：《中国肿瘤临床》2011年第23期1435-1438,共4页Chinese Journal of Clinical Oncology

基　　金：福建省卫生厅青年科研课题基金(编号:2009-2-20)资助~~

摘　　要：目的:分析ⅡA分泌型磷脂酶A2(ⅡA sPLA2)在胃癌及非癌组织中的表达,探讨ⅡA sPLA2表达与幽门螺杆菌感染(Hp)、微血管生成和胃癌临床病理特征间的相关性。方法:采用MaxVision免疫组化法检测100例进展期胃癌组织(AGC)、30例基本正常胃黏膜(NGM)、30例慢性炎症伴肠化(IM)、30例不典型增生(DYS)组织中的ⅡA sPLA2蛋白的表达情况、Hp的感染率以及CD34标记的微血管密度。结果:ⅡA sPLA2在IM、DYS及AGC中的阳性检出率分别为88.3%、66.7%及44.0%,显著高于阳性检出率为23.3%的NGM(P<0.05),但ⅡA sPLA2在AGC中的表达水平明显低于IM及DYS(P<0.05)。IM、DYS、AGC组的Hp感染率分别为43.3%、36.7%及38.0%,差异无统计学意义(P>0.05),且均高于NGM组的为13.3%Hp感染率(P<0.05)。IM及DYS组中,Hp阳性感染的组织标本的ⅡA sPLA2表达水平明显高于Hp阴性的组织标本(P<0.05),而在AGC组,Hp阳性感染与Hp阴性的标本中ⅡA sPLA2的表达差异无统计学意义(P>0.05)。ⅡA sPLA2的表达水平与AGC的浸润深度、淋巴结转移有关(P<0.05),ⅡAsPLA2高表达的AGC标本间质微血管密度明显低于ⅡA sPLA2低表达的AGC标本(P<0.05)。结论:ⅡA sPLA2参与了胃癌的发生与演化,其表达下调可能是胃癌形成的早期事件,且与Hp感染无关。ⅡA sPLA2表达水平下调可能是胃癌抗肿瘤免疫失调的一个重要环节。ⅡA sPLA2表达下调可能对胃癌的侵袭、转移以及肿瘤微血管生成存在一定的影响。Objective： To compare the expression of group Ⅱ A secretory phospholipase A2 （ Ⅱ A sPLA2 ） between gastric carci- noma and noncancerous tissues and to analyze the correlations of Ⅱ A sPLA2 expression with helicobacter pylori （ Hp ） infection, microvessel formation, and clinicopathologic characteristics. Methods： One hundred pathologic specimens from patients with advanced gastric carcinoma （ AGC ） were collected. The MaxVision immunohistochemical method was used to measure the expression of lI A sP- LA2 protein in the 100 AGC cases, in addition to 30 cases with normal gastric mucosa （NGM）, 30 with chronic inflammation plus intes- tinal metaplasia （ IM ）, and 30 with dysplasia （ DYS ）. In addition, the Hp infection rate and CD34-1abeled microvessel density were determined. Results： Expression of ]I A sPLA2 （ positive status ） was significantly higher in the IM group （ 88.3% ）, the DYS group （ 66.7% ）, and the AGC group （ 44.0 % ） than in the NGM group （ 23.3% ） （ P 〈 0.05 ）. However, the expression of ]I A sPLA2 was sig- nificantly lower in the AGC group than in the IM and DYS groups （ P 〈 0.05 ）. There were no significant differences in the rate of Hp infection among the three groups （ IM： 43.3%, DYS： 36.7%, AGC： 38.0%; P 〉 0.05 ） but Hp infection rate was significantly higher in all these groups than in the NGM group （ 13.3% ） （ P〈 0.05 ）. In the IM and DYS groups, the expression ofllA sPLA2 was higher in the specimens with Hp infection than in those without Hp infection （ P 〈 0.05 ）. In the AGC group, however, there were no statistical differences in ]l A sPLA2 expression between specimens with and without Hp infection （ P 〉 0.05 ）. Positive expression of lI A sPLA2 was correlated with the infiltration depth of AGC and nodal metastasis （ P 〈 0.05 ）. In the AGC group, the microvessel density was sig- nificantly lower in the specimens with higher H A sPLA2 expression （ P 〈 0.05 ）. Conclusion： Group

关 键 词：胃癌 磷脂酶 幽门螺杆菌 侵袭 微血管 

分 类 号：R735.2[医药卫生—肿瘤]