缺血后处理对大鼠心肌细胞凋亡及Bcl-xl/s的影响  

Effects of Ischemic Postconditioning on Cardiomycyte Apoptosis and Expression of Bcl-xl/s in Rats

作　　者：董福强[1] 田轶魁[1] 魏民新[1] 张鑫[2] 

机构地区：[1]天津医科大学总医院心血管外科,300052 [2]天津医科大学总医院内分泌实验室,300052

出　　处：《天津医药》2011年第12期1130-1132,I0010,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:81041042);天津市应用基础及前沿技术研究计划项目(项目编号:10JCZDJC19200);天津医科大学科学基金资助项目(项目编号:2009KY30)

摘　　要：目的:从Bcl-xl/s基因和蛋白水平探讨缺血后处理抗大鼠心肌细胞凋亡的机制。方法:18只健康Wistar大鼠随机分为假手术(Sham)组、缺血再灌注损伤(I/R)组、缺血后处理(PostC)组,每组6只。采用原位缺口末端标记(TUNEL)检测大鼠心肌细胞凋亡指数;免疫组化法检测BcL-xl/s蛋白表达水平;荧光实时定量PCR法检测心肌组织Bcl-xl/s基因表达水平。结果:与I/R组相比,PostC组再灌注后心肌细胞凋亡指数明显降低,PostC组Bcl-xl蛋白及mRNA表达水平明显上调,Bcl-xs蛋白及mRNA表达水平明显下调。结论:PostC能显著减少缺血再灌注后心肌细胞凋亡,凋亡相关基因Bcl-xl、Bcl-xsmRNA及蛋白参与了由缺血再灌注诱导的心肌细胞凋亡。Objective：To study the mechanism of anti-apoptosis and changed expression of Bcl-xl/s in ischemic postconditioning （PostC） in rats. Methods： Eighteen healthy adult male Wistar rats were divided into three groups including Sham group, ischemia-reperfusion （I/R） group and PostC group （n=6 for each）. TUNEL staining was used to analyse the myocardial apoptotic index. Immunohistochemisty was used to detect the protein level of Bcl-xl/s. The expressions of Bcl-xl mRNA and Bcl-xs mRNA were determined by real-time PCR. Results： Compared with I/R group, the cardiomyocyte apoptotic index significantly decreased, the expression of Bcl-xl protein and Bcl-xl mRNA increased in PostC group. The expressions of Bcl-xs protein and Bcl-xs mRNA decreased significantly in PostC group than those of I/R group. Conclusion： PostC can significantly decrease the cardiomyocyte apoptosis after I/R injury, in which Bcl-xl and Bcl-xs mRNA involved in I/R-induced myocardial apoptosis.

关 键 词：肌细胞 心脏 心肌再灌注损伤 细胞凋亡 bcl-x蛋白质 原位缺口末端标记 免疫组织化学 逆转录聚合酶链反应 大鼠 Wistar 缺血后处理 

分 类 号：R-332[医药卫生]