噪声性听力损失易感性与超氧化物歧化酶基因单核苷酸多态性的关联  

Association between SNPs in SOD1 and noise-induced hearing loss in Chinese Han population

作　　者：李旭东[1,4] 陈建雄[1] 刘移民[2,4] 苏世标[1,4] 郭晓[3,4] 

机构地区：[1]广东省职业病防治院,广州510030 [2]广州市职业病防治院 [3]广州市安全生产监督管理局 [4]中山大学公共卫生学院

出　　处：《中华劳动卫生职业病杂志》2011年第12期921-924,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（81072277）;广东省自然科学基金项目（7018635）;广东省医学科研基金（A2010050、B2009013）;广州市医药卫生科技重点项目（2006-ZDi-06）

摘　　要：目的探讨中国汉族人群中噪声性听力损失（NIHL）易感性与超氧化物歧化酶基因（SODl）rs1041740、rs2070424、rs10432782和rs4998557单核苷酸多态性（SNP）之间的关联。方法利用病例一对照研究，通过比较同一噪声暴露强度作业人员的左耳3000Hz频段听阈位移情况，筛选出听阈位移最大的10％个体作为该研究的易感人群组，共201例；听阈位移最小的10％个体作为耐受人群组，共202例，并进行相关的职业卫生调查和问卷调查。抽取易感人群和耐受人群空腹外周静脉血5ml用于常规方法抽提基因组DNA，TaqMan探针法化学荧光等位基因鉴别试验检测SNP。结果SOD1基因rs2070424A等位基因是NIHL的保护因素，与G等位基因相比，OR值为0．67，95％C1为0．50～0．88，携带AA基因型的个体患NIHL的风险是GG基因型的0．48倍，95％CI为0．26—0．79。校正混杂因素后，OR值为0，44，95％CI为0．25～0．78。结论在中国汉族人群中，NIHL易感性与SOD1 rs2070424位点SNP有关。Objective To observe the association between SNPs in SOD1 （rs1041740, rs2070424, rs10432782 and rs4998557） and noise-induced hearing loss in Chinese Han population. Methods A case- control study was used to study the effects of environmental risk factors on the susceptibility to noise induced hearing loss （NIHL） in 201sensitive workers and 202 resistant workers. A questionnaire was designed to carry out an investigation, and an occupational health survey was used to identify the occupational risk factors. Ge- nomie DNA was extracted from peripheral blood cells using standard procedures of Takara kit, and 5 ml blood was from each subject. SNPs were detected using standard procedures of TaqMan probe allele identification method. Results In SODI gene, the A allele of rs2070424 was a protective factor of NIHL, compared with the G allele （OR=0.67, 95%CI： 0.50- 0.88）. The risk with NIHL in subjects with AA genotype was significantly lower than that in subjects with GG genotype（OR=0.48, 95%CI： 0.26-0.79）. After adjusting the confusion factors, OR was 0.44 and 95%CI was 0.25-0.78. Conclusion In Chinese Han population, the SNP of rs2070424 in SOD1 may be associated with the susceptibility to NIHL.

关 键 词：噪声 职业性 超氧化物歧化酶 多态性 单核苷酸 疾病遗传易感性 

分 类 号：R135.8[医药卫生—劳动卫生]