实时定量PCR 2^(-△△Ct)法检测非小细胞肺癌HER2基因的过表达  

Detecting Overexpression Level of HER2 Gene in NSCLC by Real-time Quantitative PCR and the 2[-Delta Delta C(T)]Method

作　　者：奉水东[1] 谭红专[2] 凌宏艳[3] 袁秀琴[1] 

机构地区：[1]南华大学公共卫生学院社会医学与卫生事业管理教研室,衡阳421001 [2]中南大学公共卫生学院流行病与卫生统计学教研室,长沙410087 [3]南华大学医学院生理学教研室,衡阳421001

出　　处：《中国肺癌杂志》2011年第12期938-942,共5页Chinese Journal of Lung Cancer

基　　金：湖南省自然科学基金(No10JJ6046);南华大学博士启动基金(No2010XQD41)资助~~

摘　　要：背景与目的正确评价HER2基因表达的状况对于肿瘤的施治具有重要的指导意义。以往的评价大多基于免疫组织化学法,但结果变异性大。本研究旨在探讨实时定量PCR 2^(-△△Ct)法检测非小细胞肺癌(non-small celllung cancer,NSCLC)HER2基因表达的可行性。方法采用实时定量PCR 2^(-△△Ct)法同时检测212例肺癌组织和与其匹配的非肿瘤组织HER2基因的表达,评价过表达水平。结果肺癌组织中HER2基因的表达水平高于非肿瘤组织(T=67,P<0.05),HER2基因的过表达率为34.0%。结论实时定量PCR2^(-△△Ct)Background and objective The evaluation of the expression level of the HER2 gene for the diagnosis and treatment of tumors is usually conducted using immunohistochemical techniques.The aim of the current study is to explore the feasibility of real-time quantitative PCR and the 2[-Delta Delta C（T）]method in detecting the level of HER2 gene overexpression in non-small cell lung cancer（NSCLC）.Methods Real-time quantitative PCR and the 2[-Delta Delta C（T）] method were used to detect the level of HER2 gene overexpression in 212 lung cancer and matched non-tumor tissue specimens. Results The expression level of HER2 gene in lung cancer tissue was higher than that in the matched non-tumor tissue, with an overexpression rate of 34%.Conclusion Real-time quantitative PCR and the 2[-Delta Delta C（T）]method can be used to detect the level of HER2 gene overexpression in NSCLC.

关 键 词：实时定量PCR HER2基因 肺肿瘤 表达 

分 类 号：R734.2[医药卫生—肿瘤]