缺氧诱导因子-1α在喉癌中的表达及其与凋亡和增殖的关系  

Expression of hypoxia inducible factor-1α and its relationship to apoptosis and proliferation in human laryngeal squamous carcinoma

作　　者：俞琳琳[1] 刘洋[2] 韩军[2] 杨大章[2] 

机构地区：[1]北京煤炭总医院耳鼻咽喉科,北京100028 [2]中日友好医院耳鼻咽喉科,北京100029

出　　处：《中日友好医院学报》2011年第6期348-350,F0002,共4页Journal of China-Japan Friendship Hospital

摘　　要：目的:探讨喉鳞癌中缺氧诱导因子-1α(HIF-1α)的表达及其与肿瘤细胞凋亡和增殖的关系。方法:运用免疫组化二步法检测喉鳞癌组织中HIF-1α与增殖细胞核抗原的表达,同时用DNA末端标记法检测喉鳞癌原位细胞凋亡。结果:59例喉鳞癌组织标本中,HIF-1α阳性表达率为54.2%(32/59),显著高于喉部良性病变(P<0.05),并与肿瘤临床分期和淋巴结转移均显著相关(均P<0.05),与组织学分级无显著相关(P>0.05)。HIF-1α阳性表达组的增殖指数(55.50±16.37)显著高于阴性表达组(P<0.05),凋亡指数(3.01±1.19)低于阴性表达组,无显著性差异(P>0.05)。结论:HIF-1α在喉鳞癌的发生发展中起到一定的作用,并与肿瘤细胞的增殖有关,但与凋亡无关。Objective：To investigate the expression of hypoxia inducible factor-1α（HIF-1α）and its relationship to apoptosis and proliferation in laryngeal squamous cell carcinoma（LSCC）.Methods：Immunohistochemical method was used to detect the expression of HIF-1α and proliferating cell nuclear antigen（PCNA）.Tunel technique was used to detect in situ cell apoptosis in LSCC.Results：Fifty nine cases of LSCC samples,HIF-1α expression was 54.2%（32/59）,significantly higher than benign laryngeal lesion.The expression of HIF-1α was related with clinical stages of cancer and lymph node metastasis（P0.05）,but not related to histological grades（P0.05）.PCNA-labeling index was higher in HIF-1α positive group（55.50±16.37）than that in negative group and the difference was statistically significant（P0.05）.However,although it was lower of apptosis index in HIF-1α positive group（3.01±1.19）than that in negative group,the difference was found to have no statistical significance（P0.05）.Conclusion：HIF-1α may play a role in the carcinogenesis of laryngeal carcinoma and is related with proliferation,but has no relationship with the apoptosis of tumor cells in LSCC.

关 键 词：缺氧诱导因子-1Α 增殖细胞核抗原 凋亡 喉肿瘤 鳞状细胞癌 

分 类 号：R739.65[医药卫生—肿瘤]