Penicillium expansum脂肪酶选择性拆分外消旋萘普生  

Enantioselective Resolution of Racemic Naproxen by a Lipase from Penicillium expansum

作　　者：唐良华[1,2] 蔡志雄[1,2] 苏敏[1,2] 祝铃[1,2] 周琼[2] 吕博彦[2] 

机构地区：[1]福建师范大学工业微生物教育部工程研究中心,福建福州350108 [2]福建师范大学生命科学学院,福建福州350108

出　　处：《过程工程学报》2011年第5期828-833,共6页The Chinese Journal of Process Engineering

基　　金：国家自然科学基金资助项目(编号:81072616);福建省自然科学基金资助项目(编号:2009J01125)

摘　　要：为了实现固定化扩展青霉TS414(Penicillium expansum TS414)脂肪酶在有机相中对外消旋萘普生的高效拆分,实验考察了水分、温度、有机溶剂、酶浓度、醇结构和醇浓度对酶促拆分反应的影响,确立了优化的酯化反应条件为:异辛烷为溶剂,外消旋萘普生2.15mmol/L,正丙醇34.3mmol/L,固定化酶量12g/L,水0.05%(φ),40℃恒温摇床中200r/min反应100h.在此条件下,酯化拆分反应的转化率为48.3%.结果表明,固定化Penicilliumexpansum脂肪酶是一种较为理想的用于外消旋萘普生拆分的工具酶.In order to resolute racemic naproxen with high efficiency by the immobilized Penicillium expansum TS414 lipase in organic solvent,the factors affecting the resolution of（R,S）-naproxen by lipase-catalyzed esterification reaction were investigated,including water concentration,temperature,organic solvent,enzyme concentration,structure and concentration of alcohol.And experiments were designed to optimize the conditions of esterification reaction.Under the optimal condition（isooctane as solvent,2.15 mmol/L ibuprofen,12 g/L lipase,34.3 mmol/L 1-propanol,0.05%（j） water,200 r/min,40℃ for 100 h）,the conversion rate could reach 48.3%.Thus,Penicillium expansum TS414 lipase could be used as a perfect tool enzyme with high enantioselectivity and had a great potential for commercial applications in the resolution of racemic naproxen and other non-steroidal anti-inflammatory drugs of 2-aryl propionic Acids.

关 键 词：脂肪酶 萘普生 拆分 

分 类 号：Q93[生物学—微生物学]