环境设置对局灶性脑梗死大鼠梗死灶周围神经丝蛋白、突触膜糖蛋白表达的影响  

Effect of environmental intervention on neurofilament and synaptophysin expression in rats after unilateral local cerebral infarction

作　　者：李娜[1] 陈景红[1] 高俊淑[1] 李阔 贾子善[3] 

机构地区：[1]河北省人民医院神经内科,石家庄050051 [2]沧州市地区医院神经内科,061001 [3]河北省人民医院康复中心,石家庄050051

出　　处：《疑难病杂志》2011年第12期919-921,F0003,共4页Chinese Journal of Difficult and Complicated Cases

摘　　要：目的观察学习环境对局灶性脑梗死大鼠神经丝蛋白(NF)、突触膜糖蛋白(Syn)表达的影响。方法 SD大鼠110只,随机分为假手术对照组(对照组,10只)和大脑中动脉阻塞(MCAO)组(100只)。MCAO组大鼠采用电凝法造成右侧MCAO模型后,再随机分为独居亚组(30只)、社会交往亚组(社交亚组,40只)和探索学习亚组(探索亚组,30只)。于术后7d、14d、21d、28d分批处死大鼠,用免疫组化染色观察梗死灶周围皮质NF、Syn阳性表达。结果 MACO后7d不同环境设置亚组动物NF的表达水平均低于对照组(P<0.05),但其表达逐渐增高,28d时,探索亚组、社交亚组高于对照组(P<0.05);MCAO后7d、28d不同环境设置亚组梗死灶周围皮质Syn阳性表达均高于对照组(P<0.05);探索亚组于MCAO术后各时间点梗死灶周围皮质NF、Syn表达均明显优于独居亚组和社交亚组(P<0.05,P<0.01);第7天、21天、28天社交亚组NF表达均优于独居亚组(P<0.05),第7天、14天、21天社交亚组Syn表达均优于独居亚组(P<0.05)。结论探索学习环境、社会交往环境能促进梗死灶周围NF、Syn的表达。Objective To observe effects of different environmental intervention on neurofilament（NF） and synaptopliysin （Syn）expression in rats after unilaleral local cerebral infarction.Methods 110 SD rats were randomly divided into sham（n=10） and middle cerebral artery occlusion（MCAO） groups（n=100）.The rats in MCAO group were subjected to electric coagulation of MCA and then were assigned randomly to three subgroup,individual living subgroup（n=30）,social communication subgroup（n=40）,learning subgroup（n=30）.The rats were randomly sacrificed at different time points（7 d,14 d,2l d,28 d alter operation）.The expression of NF and Syn in peri-ischemic cortex was examined by immunohistochemistry staining.Results After MCAO,the expression of NF and Syn in learning subgroup was higher than other subgroups at any time point（P0.05,P0.01）.In addition,the expression of NF in social communication subgroup was stronger that in individual living subgroup on day 7,21 and 28（P0.05）,the expression of Syn in social communication subgroup was stronger that in individual living subgroup on day 7,14 and 21（P0.05）.Conclusion Learning and social communication environment after MCAO could up-regulate the expression of NF and Syn in the peri-ischemic regions.

关 键 词：脑梗死 局灶性 探索学习 神经丝蛋白 突触膜糖蛋白 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]