索坦对肾透明细胞癌786-0细胞生长与Tiam1表达的影响  

Effect of sunitinib on cell growth and Tiam1 expression in human renal carcinoma cell line 786-0

作　　者：孟庆泽[1,2] 乔保平[1] 宫璀璀[3] 刘德海[2] 张喜卿[4] 

机构地区：[1]郑州大学第一附属医院泌尿外科,郑州450052 [2]中国人民解放军153中心医院泌尿外科,郑州450042 [3]中国人民解放军153中心医院中心实验室,郑州450042 [4]中国人民解放军153中心医院病理科,郑州450042

出　　处：《郑州大学学报（医学版）》2011年第6期865-868,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省教育厅科技攻关基金资助项目2006320047

摘　　要：目的:探讨索坦对肾透明细胞癌786-0细胞生长与Tiam1表达的影响。方法:将对数生长期肾透明细胞癌786-0细胞分为4组:索坦低、中、高浓度处理组和正常对照组。索坦低、中、高浓度处理组细胞分别用2、4和8μmol/L索坦培养24、48和72h。采用免疫细胞化学和原位杂交技术检测各组细胞中Tiam1蛋白和mRNA的表达。采用MTT和TUNEL法检测培养48h各组细胞凋亡和存活情况。结果:各组细胞培养24、48和72h时间点的Ti-am1蛋白及mRNA表达比较,差异有统计学意义(F=898.782、551.191、510.484,294.803、460.127、358.019,P均<0.05)。索坦高、中、低浓度处理组不同时间点Tiam1蛋白及mRNA表达比较,差异有统计学意义(F=38.601、81.324、262.601,120.671、509.902、56.464,P均<0.05)。同一时间点随着索坦浓度增加,细胞Tiam1蛋白及mRNA的表达减弱(P<0.05);同一浓度处理组中,随着时间延长,Tiam1蛋白及mRNA的表达降低(P<0.05)。培养48h,各组细胞存活率和凋亡率比较,差异有统计学意义(F=123.432和120.341,P均<0.001),随着索坦浓度增加,细胞存活率降低,凋亡率增加(P<0.05)。结论:索坦可能通过抑制Tiam1基因在肾癌细胞株786-0中表达,促进肿瘤细胞凋亡,抑制细胞生长。Aim：To explore the effect of sunitinib on the cell growth and Tiam1 expression in human renal carcinoma cell line 786-0.Methods：786-0 cells were divided into four groups：sunitinib low,mid,and high concentration groups（2,4,8 μmol/L sunitinib were used to treat 786-0 cells for 24,48 and 72 h,respectively）,and control group.Cell cycle and apoptosis rate in each group was detected by MTT assay and TUNEL after 48 h treatment.Immunocytochemistry and in situ hybridization were used to detect the expressions of Tiam1 protein and mRNA after 24,48,and 72 h treatment.Results：The expressions of Tiam1 protein and mRNA among the four groups at 24,48,72 h had significant difference（F=898.782,551.191,510.484,294.803,460.127,358.019,P0.05）.The expressions of Tiam1 protein and mRNA at different time points had significant difference in the sunitinib low,mid,or high concentration group（F=38.601,81.324,262.601,120.671,509.902,56.464,P0.05）.At the same time point,the expressions of Tiam1 decreased with the increased concentration of sunitinib.And in the same group,the expressions of Tiam1 decreased with time.After being cultured for 48 h,the survival rate and apoptosis rate of the cells among the four groups had significant differences（F=123.432,120.341,P0.001）,and the survival rate decreased and the apoptosis rate increased with the increased concentration of sunitinib.Conclusion：Sunitinib may promote renal carcinoma cell apoptosis and inhibit the cell growth through decreasing the expression of Tiam1.

关 键 词：786-0细胞系 TIAM1 肿瘤转移 索坦 凋亡 

分 类 号：R737[医药卫生—肿瘤]