响应面分析法优化微波提取密蒙花总黄酮工艺  

Optimization of Microwave Extraction Technology for Flavonoids from Buddleja Officinalis Maxim with Response Surface Method

作　　者：罗堾子[1,2] 张弘[1] 郑华[1] 孔永强 张加研[2] 郭元亨[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,昆明650224 [2]西南林业大学木质科学与装饰工程学院,昆明650224 [3]景谷林化有限公司,云南景谷666400

出　　处：《中国食品学报》2011年第8期79-86,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：科技部农业科技成果转化资金项目(2010GB24320619);国家林业科技成果推广项目

摘　　要：以水为介质,密蒙花总黄酮提取率为考察指标,优化微波强化提取密蒙花总黄酮的工艺。选取微波功率、微波工作时间和液料比为影响因子,在单因素试验的基础上,进行3因素3水平的Box-Behnken中心组合试验设计。以密蒙花总黄酮提取率为响应值,进行响应面(RSM)分析,优化微波强化提取密蒙花总黄酮的提取条件。试验结果表明,微波处理密蒙花有利于总黄酮的提取,微波提取最佳条件为:提取功率600W、提取时间12min、液料比45∶1(mL:g)。在最佳的提取条件下,密蒙花总黄酮提取率理论值可达5.48%,验证值为5.49%,验证值与理论值间的相对误差为0.18%。The extraction process of Buddleja officinalis Maxim Flavonoids was found with microwave method, which used water as medium and the extraction rate of Buddleja officinalis Maxim Flavonoids as index. Then select the microwave power, extracting time and ratio of water to solid as random factor, on the basis of single factor experiment, 3 factors 3 levels of Box-Behnken center combination experimental design, the extraction rate of Buddleja officinalis Maxim flavonoids were used as response value for response surface （RSM） analysis, and the flavonoids microwave extraction conditions were optimized. The experimental results showed that the flower treatment with microwave is useful for flavonoids leaching. The best extraction conditions as follows, microwave power 600 W, extraction time 12 min, and the ratio of water to solid 45∶1. Under the best extraction conditions, the theoretical extraction rate of Buddleja officinalis Maxim flavonoids can reach 5.48%, validation value is 5.49%, the relative error between theoretical and validation is 0.18%.

关 键 词：微波提取 密蒙花 总黄酮 响应曲面 

分 类 号：TQ461[化学工程—制药化工]