穴位埋线治疗实验性结肠炎大鼠的机制探讨  

On Mechanism of Acupoint Embedment Treatment for Rats with Colonitis

作　　者：张夏毅[1] 沈霖[1] 范恒[1] 梁丽[1] 廖弈[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院中西医结合科

出　　处：《世界中医药》2011年第6期527-529,共3页World Chinese Medicine

基　　金：国家自然科学基金项目(NO:30772878)

摘　　要：目的:检测TNBS诱导实验性结肠炎大鼠在穴位埋线治疗前后的NF-κBp65和STAT6mRNA的表达水平,初步阐明穴位埋线治疗实验性结肠炎大鼠的作用机理。方法:18只雄性大鼠随机分为正常组、模型组、穴位埋线组,每组6只。除正常对照组未行造模外,其余2组大鼠均采用TNBS造模。模型组不设干预,正常饮食;穴位埋线组进行穴位埋线治疗。治疗15d后观察大鼠的结肠病理组织学改变,用western blot法检测大鼠脾淋巴细胞NF-κBp65蛋白的表达;用RT-PCR法检测大鼠结肠组织STAT6mRNA的表达。结果:穴位埋线组的大鼠腹泻、黏液脓血便症状得到较快改善,大鼠黏膜组织损伤也明显改善。与正常组相比,模型组大鼠NF-κBp65和STAT6mRNA增多(P<0.01);与模型组比较,穴位埋线组NF-κBp65和STAT6mRNA减少(P<0.01)。结论:穴位埋线能通过NF-κB和STAT6双信号途径下调炎症因子,从而发挥治疗作用。Objectives：To determine the expression of NF - KBp65 and STAT6mRNA prior to and after acupoint embedment treatment in rats with TNBS induced colonitis, and to investigate mechanism of action of the treatment. Methods：The male rats （n = 18 ） were randomized to normal group, model group and treatment group. The model was established using TNBS. The model group received no intervention, and was on normal diet. aeupoint embedment treatment. On D15 the histopathology of colon was observed, NF -KBp65 expression was determined by western blot, and STAT6mRNA expression was determined by RT - PCR. Results ： The treatment group recovered more fast from diarrhea and bloody purulent stool, and mucous membrane injury was significantly improved. Compared with the normal group, the model group had significantly higher expression of NF - KBp65 and STAT6mRNA （P 〈0.01 ） , while the treatment group had significantly lower expression （P 〈 0.01 ）Conclusion：The effectiveness of acupoint embedment treatment is achieved by down regulation of inflammatory factor through NF - KBp65, STAT6mRNA.

关 键 词：结肠炎 穴位埋线 NF-ΚBP65 STAT6 

分 类 号：R574.62[医药卫生—消化系统]