响应面法优化枸杞多糖提取的研究  

Study on Optimization of the Extraction Technology of Lycium Barbarum Polysaccharide by Response Surface Methodology

作　　者：刘琳[1] 鄢瑞明[1] 曾凡骏[1] 

机构地区：[1]四川大学食品科学与工程系,成都610065

出　　处：《食品与发酵科技》2011年第6期32-35,共4页Food and Fermentation Science & Technology

摘　　要：实验采用热水浸提法提取多糖,以蒽酮—硫酸法在620nm下检测枸杞多糖的含量,计算出得率。采用Box–Behnken统计设计技术优化枸杞多糖提取方法,得到的最佳工艺条件是:提取温度87℃,料液比1:26,提取时间7h,一次提取枸杞多糖得率最高可达8.2%。不仅得出了枸杞多糖的最佳提取条件,而且采用响应面设计实验得到的结果相对传统的正交实验准确度更高,为以后大规模工厂化生产提供了参考。In this study,the method of polysaccharide extraction was hot water extraction and discussing the crushing of raw materials,decolor,pre-soaked in the three factors of pre-experimental extraction to the effect of the follow-up.With anthrone-sulfuric acid method,the content of LBP was tested under the 620nm and the yield was calculated.Box-Behnken statistical design technology was used to optimize the method of LBP extraction.With the response surface optimization,the optimum conditions were： extraction temperature 87℃,liquid ratio 1：26,extraction time 7h.On the condition,the highest yield of LBP was 8.2%.The results were not only had the best extraction conditions for LBP,but also the results of response surface design experiment was much higher the traditional orthogonal in the degree of accuracy.The reference was provided for flarge-scale factory production in the future.

关 键 词：枸杞 多糖 检测 提取 响应面 

分 类 号：Q539[生物学—生物化学]